英文名称： Tumor Necrosis Factor-alpha (TNF-α) (Human)
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产品特点：
1、万级GMP自动化生产车间生产，严格遵守ISO国际质控标准；
2、高纯度、高活性；
3、无热源、无内毒素、无外源因子。
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人肿瘤坏死因子-α 产品说明书
产品描述:
Tumor necrosis factor alpha (TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and crossreactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
表达系统 :
Escherichia coli
溶解方法:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C Further dilutions should be made in appropriate buffered solutions.
产品组成:
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
存储条件:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
分子量:
Approximately 18.3 kDa, a single, non-glycosylated polypeptide chain containing 157 amino acids with 6 × His at N-terminus.
内毒素:
Less than 1 EU/μg of TNF-α, His as determined by LAL method.
产品用途:
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.
生物学活性:
The ED50 determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D.
产品形态:
Sterile filtered white lyophilized (freeze-dried) powder.
氨基酸序列:
MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL
产品纯度:
> 97% by SDS-PAGE and HPLC analyses.