Taq Enzyme

    PCR  Reaction Kit  Components:

         (reagents are sufficient  for 200  reactions of 50ul volume)

                  Taq enzyme(5units/ul)                                                                      50uL

                  dNTP(2.5mM of dATP  dCTP  dGTP and dTTP)                          800uL

                 10Xreaction buffer (15mM MgCI2)                                                  1mL

     Applications

                 1.polymerase  chain  reaction  to  amplify  DNA

                2. Sequencing

  Typical PCR Reaction  Mixture:

          (for  a   50uL  reaction)

                Taq   enzyme(5unit/uL)                                                                  0.25uL

               Template   DNA                                                                              <200ng

               10Xbuffer                                                                                         5uL

               dNTP(2.5mM   of     each)                                                               4uL

               Primers                                                                                              10-50pmol  of  each 

               Nuclease-free  water                                                                           to   50uL