Progenesis MALDI
型号:: 2.6
价格:请致电:010-57128832,18610462672
品牌: 英国    产品商标: nonlinear dynamics

利用生成的高质量蛋白质谱数据,Progenesis MALDI可以从各种精度的MALDI-TOF质谱(包括SELDI)中发现生物标志物。因为采用了独一无二的Progenesis技术方案,Progenesis MALDI不仅提高了实验结果的可靠性和重复性,它还让用户的生物标志物发现更为高效、客观且具有很强的统计性。



高效性

Progenesis MALDI能够分析来自单一实验的数百张谱。而运用这种快速、简单、大批量数据分析的模式,将易于分析足够多样本,从而可以控制假阳性发现率并增加分析结果的可行性。



客观性

根据一系列客观的工作流程,Progenesis MALDI将同样的特征在所有谱中进行统一检测,随后,再将检测结果进行自动应用统计分析,以帮助用户在不同实验及不同实验室中均能重现实验结果。



统计性

Progenesis MALDI能够结合实验设计中技术及生物学因素进行合理分组,并展开正确的分析。另外,谱比对及一致性特征检测产生的完整数据集,又有助于用户进行多变量统计分析。



解决用户的分析问题

“Progenesis MALDI能够帮助我利用MALDI-TOF质谱里的完整细胞谱系进行各种不同的分析。现在,我可以快速地从一大批样本中进行谱比对。再结合强大的统计分析方法(如ANOVA, PCA), 帮助我发现新的生物标志物。更棒的是,每个使用者都可以轻松掌握这个工具。如果用户和我有相似的需求,我强烈推荐Progenesis MALDI。”

----Valerie Labas, Plate-forme d’Analyse Integrative de Biomarqueurs cellulaires et moleculaires, INRA de Tours, Nouzilly, France

r:  female  CRL-1739 AGS 人胃腺癌细胞
Ethnicity:  Caucasian  
Comments: The AGS cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy. The cells have a plating efficiency of 34% in the medium below. The line was cured at the ATCC of a prior mycoplasma infection .Subsequently, AGS has been determined to be infected with Parainfluenza type 5 (PIV5 formerly known as SV5). [PubMed: 17509637] 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Subculturing:  Protocol: 
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.CRL-1739 AGS 人胃腺癌细胞

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended 
Medium Renewal: Every 2 to 3 days 
Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase 
Doubling Time:  20 hrs  
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020 
References: 22748: Barranco  
 CRL-1739 AGS 人胃腺癌细胞