英文名称： flocel Blood-brain barrier in vitro
产品详细描述
详细配制内容及参数资料
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TEER检测系统
4个DIV-BBB盒子
DIV-BBB?管腔
特性 | 优点 |
可控制腔内/非腔体积比 体型小，仅7cm长 电极与管腔一体化 成本低 | 与体内体积比相符 减少了试验所需细胞数目 TEER检测系统操作简单 一次性使用，不能重复使用 |
经毛细管孔径：0.2um
中空纤维数量：19
腔内总体积
腔内表面积
腔外体积
腔外中空纤维总面积：3.50 in3= 22.6cm2
TEER检测系统
TEER检测系统为研发人员提供了一个快速、简便地评估血脑屏障完整性方法，此外，电阻抗值（＞1000
特性 | 优点 |
多频率下检测电阻抗 | 描述电极的电阻和电容 |
低电压，Max=60毫伏 | 限制对屏障的潜在破坏作用 |
自动检测多个管腔 | 可同时连接4个盒子 |
检测软件可自定义设置泵的流速、频率、波形等参数 | 检测电阻抗，记录数据 |
USB连接 | 台式或者便携式电脑均可连接 |
脉动泵
脉搏率和作用周期完全模拟体内血液动力学设计，贴近体内环境，试验准确，脉搏率可设置在30-120/分范围内。其特性和优点见下：
特性 | 优点 |
4个管腔的流速和泵的速度可分别控制， | 允许4个独立试验 |
外部24电压墙与电源连接 | 减少培养箱中高温 |
接受来自TEER检测系统的指定程序 | 不需要额外的USB接口 |
电脑配置最低要求
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About Flocel
Every year millions of dollars are spent by pharmaceutical companies to develop and test drugs using in vitro models of the Blood-Brain Barrier (BBB). Many of these cases end up not working in vivo.CleveMedhas licensed technology fromThe Cleveland Clinic Foundation
Flocel also offers atesting service for BBB permeability compounds. To learn more about our testing services, clickhere.
Introduction to the Blood-Brain Barrier
TThe blood-brain barrier (BBB) explains why certain substances circulating in the blood are able to get into the brain and others do not. The BBB is formed by brain capillaries that look like simple tubes of endothelial cells. Experimental and clinical evidence suggests that the BBB maintains the chemical environment for neuronal function and protects the brain from harmful substances. The blood-brain barrier is therefore crucial for complex issues such as drug delivery, pathogenesis of chronic neurological diseases, and issues related to bio-defense. We have developed a practical in vitro model that accurately reflects BBB characteristics.
Benefits of Flocel’s in vitro BBB Model
In contrast to other available models of the blood-brain barrier, Flocel's Dynamic In vitro Blood-Brain Barrier (DIV-BBB) respects the anatomical aspects of the in situ endothelial cell-astrocyte interactions and replicates the physiological levels of shear stress to which in situ endothelial cells are exposed. The DIV-BBB allows formation of physiological transendothelial resistance, and formation of gap junctions that can be easily visualized at the electron microscope or determined experimentally with the use of tracers.
Mission
Flocel Inc. is a biotechnology company dedicated to innovation towards helping the research community better conduct and advance in vitro studies.
In vitro BBB Technology
The blood-brain barrier (BBB) explains why certain substances circulating in the blood are able to get into the brain and others do not. As determined by electron microscopy, the BBB is formed by brain capillaries that look like simple tubes of endothelial cells linked by tight junctions. The Flocel in vitro BBB is a tool that allows to reproduce in a flow-based 3D cell culture apparatus the essential properties of the human BBB, including the expression of metabolic and extrusion mechanisms responsible for multiple drug resistance to antiepileptic drugs, anti-tumor agents, etc.
The Flocel apparatus consists of hollow fibers (the artificial blood vessels) that are surrounded by an extracapillary space (the brain side). In addition to the cerebrovascular system, this apparatus can also be used to mimic the bone marrow, the placental barrier, or the CSF-blood barrier. In the case of modeling of bone marrow, the abluminal space is an ideal environment for the growth and differentiation of stem cells.
The Flocel DIV-BBB package contains: in vitro cell culture cartridges, a computer-controlled perfusion system, and a trans-endothelial electrical resistance (TEER) measurement module.
In vitro Cell Culture Module
In contrast to other available models of the blood-brain barrier, the DIV-BBB respects the anatomical aspects of the in situ endothelial cell-astrocyte interactions and allows the formation of tight junctions that can be easily visualized at the electron microscope or determined experimentally with the use of tracers. Cell differentiation is also crucially modulated by exposure to shear stress.
Features:
? Controlled ratio of luminal to ablumenal volume.
? Small size, only 2.75 inches long.
? Electrodes built into the cartridge.
Benefits:
? Matches volume ratio found in vivo.
? Reduces the number of cells required.
? TEER measurements are easily made.
? One-time use, no rebuilding of the cartridge.
? Low cost.
Specifications:
? Hydrophobic capillaries Accurel? PP Q3/2 (optionally pre-coated with ProNectin? For Poly-DLysine)
? Trans-capillary pore size nominal: 0.2 μm
? Number of hollow fibers: 19
? Total lumen internal volume: 0.0123 in3= 0.202 cm3
? Total lumen internal surface area: 2.09 in2= 13.5 cm2
? Volume of extralumenal space (ECS): 0.070 in3= 1.15 cm3
? Total hollow fiber area in ECS: 3.50 in2= 22.6 cm2
? Cartridges are delivered in a sealed bag, sterile and wetted with distilled water.
Download theDIV-BBB Cartridge Brochure
This brochure requires Acrobat or Adobe Reader 6.0 software to open.
Trans-endothelial Electrical Resistance (TEER) Measurement Module
The Trans-Endothelial Electrical Resistance (TEER) measurement provides a quick and easy evaluation of the integrity of the Blood-Brain Barrier model. The DIV-BBB model has a TEER closer to that found in vivo, >1000 Ω-cm2as opposed to the monolayer model that typically achieves a TEER of 200 Ω-cm2.
Features:
? Impedance measurement at multiple frequencies.
? Low voltages, 60 millivolts maximum.
? Automatic multiplexing of multiple cartridges.
? Two versions, PCI card or PCMCIA card.
Benefits:
? Characterize the resistance and capacitance of the barrier.
? Limits potentially damaging voltages to the barrier.
? Can handle up to 4 cartridges simultaneously.
? Can use either a desktop or laptop PC.
Computer System Requirements
? IBM compatible computer, 400 MHz or faster
? Windows 98SE or higher
? 128 MB RAM (256 MB recommended)
? CD-ROM drive for installation
? One free USB port
The TEER Measurement System is sold with the measurement software, the required cable, and a mounting platform that can accommodate four DIV-BBB cartridges. The electrodes in the cartridge plug into the mating connectors in the platform. The system is shipped with four cartridges for initial evaluation.
Computer-controlled Perfusion System
The Flocel Quad Pump mimics the pulsitile action of the heart in this model. The Quad Pump contains four small, easily configurable, pulsitile pumps that allow continuous flow through the DIV-BBB model. Each pump is individually controlled, allowing up to four independent experiments to be conducted at one time.
Features
? Flow rates and pump rates are independently controlled for four cartridges.
? External 24 VDC wall plug-in power supply.
? Receives program commands from TEER measurement system.
Benefits
? Allows for four independent experiments.
? Minimizes heat inside incubator.
? Does not need an additional USB port.
Specifications for each of the four Linear Actuators
? Pump rate: 30-120 bpm
? Flow rate: 1-50 ml/min
? Power Cable: Controlled by TEER
? External +24 vdc Supply
? Control Cable: Controlled by TEER
? Power consumption: 7.5 W
? Weight: 5.8 oz
The Quad pump and its casing is made of the plastic Delrin; the pump also comes with its own tubing.
