上皮细胞电阻仪
型号:: RE1600
价格:请致电:010-57128832,18610462672
品牌: 中国    产品商标: kingtech

RE1600上皮细胞电压电阻仪是手持式的测量仪器,由电阻测量仪及电极组成。测量系统采用交流脉冲技术,避免了电极上的金属沉积以及持续的直流电流对组织的副作用,电阻读数不受膜电容和膜电压影响。内置1K欧姆的标准电阻,通过按钮可以随时检查仪器测量电路是否正常。

测量电极采用双腿四电极结构,内侧为一对电压电极,外侧为一对电流电极。两腿的长度不同,测量时较短的腿插入培养杯中,较长的腿插入培养杯外,选择合适的量程,即可实时显示测量值。

RE1600上皮细胞电压电阻仪也可以用来测量组织的跨膜电压。

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  • 用电力方法检测了血脑屏障的完整性

  • 可以用真实的细胞做实验

  • 比现存的静态模型更能形成紧密的连接

  • 降低药物研发的成本

  • 计算机控制灌流泵,智能地调整液流

  • 1.2 配置及参数资料
    TEER检测系统 CARTRIDGE底板 
    4个DIV-BBB 盒子 检测软件 电缆线 
    DIV-BBB?管腔


    特性

    优点

    可控制腔内/非腔体积比 
    体型小,仅2.57cm长 
    电极与管腔一体化 
    成本低

    与体内体积比相符 
    减少了试验所需细胞数目 
    TEER检测系统操作简单 
    一次性使用,不能重复使用

    疏水性毛细血管(Hydrophobic capillaries) Accurel PP Q3/2 (optionally pre-coated with ProNectin

    For Poly-D-Lysine)
    经毛细管孔径: nominal 0.2um
    中空纤维数量:19
    腔内总体积 : 0.0123in3 = 0.202cm3
    腔内表面积 : 2.09 in3= 13.5cm2
    腔外体积 :0.070 in3 = 1.15cm3
    腔外中空纤维总面积: 3.50 in3 = 22.6cm2
    跨细胞膜电阻测量(TEER 检测)系统 
    TEER检测系统为研发人员提供了一个快速、简便地评估血脑屏障完整性方法,此外,电阻抗值(>1000 -cm2)也非常接近体内数值。

    TEER检测系统同时配带了所需检测软件、电线和衬板,可供4个DIV-BBB培养管腔同时使用。其中检测软件可以自定义设置泵的流速、

    频率、波形等参数。其它特性及优点见下表:


    特性

    优点

    多频率下检测电阻抗

    描述电极的电阻和电容

    低电压,Max=60毫伏

    限制对屏障的潜在破坏作用

    自动检测多个管腔

    可同时连接4个盒子

    检测软件可自定义设置泵的流速、频率、波形等参数

    检测电阻抗,记录数据

    USB连接

    台式或者便携式电脑均可连接

    Flocel体外动态血脑屏障系统系统
    计算机控制的脉动泵 
    脉搏率和作用周期完全模拟体内血液动力学设计,贴近体内环境,试验准确,脉搏率可设置在30-120/分

    范围内。

    其特性和优点如下:


    特性

    优点

    4个管腔的流速和泵的速度可分别控制,

    允许4个独立试验

    外部24电压墙与电源连接

    减少培养箱中高温

    接受来自TEER检测系统的指定程序

    不需要额外的USB接口

    泵速: 30-120bpm
    流速: 1-50ml/min
    电源: TEER控制

    系统组成部件:

    Publications 文献

    1.The role of shear stress in Blood-Brain Barrier endothelial physiology
    Cucullo L, Hossain M, Puvenna V, Marchi N, Janigro D.

    2.>A dynamic in vitro BBB model for the study of immune cell trafficking into the central nervous system
    Cucullo L, Marchi N, Hossain M, Janigro D.

    3.>Pattern of P450 expression at the human blood-brain barrier: roles of epileptic condition and laminar flow.

    Ghosh C, Gonzalez-Martinez J, Hossain M, Cucullo L, Fazio V, Janigro D, Marchi N.

    4.>Tobacco smoke: a critical etiological factor for vascular impairment at the blood-brain barrier.
    Hossain M, Sathe T, Fazio V, Mazzone P, Weksler B, Janigro D, Rapp E, Cucullo L.

    5.>Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for

    rational neurovascular studies
    Cucullo L, Couraud PO, Weksler B, Romero IA, Hossain M, Rapp E, Janigro D.

    6.>Side by side comparison between dynamic versus static models of blood-brain barrier in vitro: a permeability

    study. 
    Santaguida S, Janigro D, Hossain M, Oby E, Rapp E, Cucullo L.

    7.>Development of a humanized in vitro blood-brain barrier model to screen for brain penetration of antiepileptic

    drugs.
    Cucullo L, Hossain M, Rapp E, Manders T, Marchi N, Janigro D.

    8.>Drug delivery and in vitro models of the blood-brain barrier
    Cucullo L, Aumayr B, Rapp E, Janigro D.

    9.Glycerophosphoinositol and *** improve transendothelial electrical resistance in an in vitro study

    of the blood-brain barrier.
    Cucullo L, Hallene K, Dini G, Dal Toso R, Janigro D.

    10.>Dynamic in vitro model of the blood-brain barrier. Gene profiling using cDNA microarray analysis.
    Marroni M, Kight KM, Hossain M, Cucullo L, Desai SY, Janigro D.

    11.>A new dynamic in vitro model for the multidimensional study of astrocyte-endothelial cell interactions at

    the blood-brain barrier.
    Cucullo L, McAllister MS, Kight K, Krizanac-Bengez L, Marroni M, Mayberg MR, Stanness KA, Janigro D.

    12.>Mechanisms of endothelial survival under shear stress.
    Desai SY, Marroni M, Cucullo L, Krizanac-Bengez L, Mayberg MR, Hossain MT, Grant GG, Janigro D.

    Posters

    1.A Dynamic Humanized drug resistant In Vitro Blood-Brain Barrier Model to assess the permeability of relevant

    CNS drugs

    2.Development of a novel blood-brain barrier model by coupling Immortalized human brain endothelial cells with

    flow-based cell culture technologies

    3.Glioblastoma Cell Line Metabolism Under Dynamic and Static Conditions: Effects on tumor growth and BBB integrity

    4.Humanized in vitro blood-brain barrier models to screen for brain penetration of antiepileptic drugs

    5.Effect of Shear stress on BBB endothelial cells: A proteomic study by 2-dimensional protein electrophoresis