食蟹猴TP53 cDNA基因克隆
英文名称： Cynomolgus monkey TP53 cDNA gene Clone / ORF gene clone
型号:null    产品货号： KG90001-G
价格:请致电：010-57128832,18610462672
品牌: 北京

官方网址：http://www.sinobiological.cn/p53-TP53-cDNA-Clone-g-6057.html
 
TP53 cDNA Clone Datasheet
 
 
 
 
 
 
 
TP53 cDNA基因克隆产品信息
 
  
  
cDNA基因长度: 
 		  
1182
 		 
 
 别名:   
TP53
 		 
 
 种属: Cynomolgus monkey 
  
 
TP53 cDNA基因克隆产品描述
 
  
  
cDNA基因描述: 
 		  
ORF Clone of? Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) tumor protein p53 DNA.
 		 
 
 克隆载体: pGEM-T vector 
 
 序列信息:  
Identical with the Gene Bank Ref. ID sequence except the point mutation 246 G/A not causing the amino acid variation.
 		 
  
 
TP53 cDNA基因克隆邮寄信息
 
 
     
  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
    2.  
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
    3.  
 
 
TP53 cDNA基因克隆使用方法
 
 
     
  1. Remove the marked edge in the center of the spot.
    2.  
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
    3.  
  3. Transfer the tube to a heating block at 95oC for 15–30 min .
    4.  
  4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap, the sample approximately 60 times.
    5.  
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
    6.  
 
 
TP53 cDNA基因克隆序列以及质控指标
 
基因序列:
 
 
 1 ATGGAGGAGC CGCAGTCAGA TCCTAGCATC GAGCCCCCTC TGAGTCAGGA AACATTTTCA 61 GACCTATGGA AACTACTTCC TGAAAACAAC GTTCTGTCCC CCTTGCCGTC CCAAGCAGTG 121 GATGATTTGA TGCTGTCTCC AGACGATCTT GCACAATGGT TAACTGAAGA CCCAGGTCCA 181 GATGAAGCTC CGAGAATGTC AGAGGCTGCT CCCCCCATGG CCCCCACACC AGCAGCTCCT 241 ACACCAGCGG CCCCTGCACC AGCCCCCTCC TGGCCCCTGT CATCCTCTGT CCCTTCCCAG 301 AAAACCTACC ATGGCAGCTA CGGTTTCCGT CTGGGCTTCC TGCATTCTGG AACAGCCAAG 361 TCTGTGACTT GCACGTACTC CCCTGACCTC AACAAGATGT TTTGCCAGCT GGCCAAGACC 421 TGCCCCGTGC AGCTATGGGT TGATTCCACA CCCCCACCCG GCAGCCGCGT CCGCGCCATG 481 GCCATCTACA AGCAGTCACA GCACATGACG GAGGTCGTGA GGCGCTGCCC CCACCATGAG 541 CGCTGCTCAG ACAGCGATGG ACTGGCCCCT CCTCAGCACC TTATCCGAGT GGAAGGAAAT 601 TTGCGTGTGG AGTATTCGGA TGACAGAAAC ACTTTTCGAC ATAGTGTGGT GGTGCCCTAT 661 GAGCCGCCTG AGGTTGGCTC TGACTGTACC ACCATCCACT ACAACTACAT GTGTAACAGT 721 TCCTGCATGG GCGGCATGAA CCGGAGGCCC ATCCTTACCA TTATCACACT GGAAGACTCC 781 AGTGGTAATC TACTGGGACG GAACAGCTTT GAGGTGCGAG TTTGTGCCTG TCCTGGGAGA 841 GACCGGCGCA CAGAGGAAGA GAATTTCCGC AAGAAAGGGG AGCCTTGCCA CCAGCTGCCC 901 CCTGGGAGCA CTAAGCGAGC ACTGCCCAAC AACACCAGCT CCTCTCCCCA GCCAAAGAAG 961 AAACCACTGG ATGGAGAATA TTTCACCCTT CAGATCCGTG GGCGTGAGCG CTTCGAGATG 1021 TTCCGAGAGC TGAATGAGGC CTTGGAACTC AAGGATGCCC AGGCTGGGAA AGAGCCAGCG 1081 GGGAGCAGGG CTCACTCCAG CCACCTGAAG TCCAAGAAGG GGCAATCTAC CTCCCGCCAT 1141 AAAAAATTCA TGTTCAAGAC AGAGGGGCCT GACTCAGACT GA
 
 
 
质控指标:cDNA sequencing results is available upon request.