Leptin receptor,瘦素受体抗体

产品编号HZ-0961M
英文名称Leptin receptor
中文名称瘦素受体抗体
别 名obl; CD 295; CD295; CD295 antigen; Db; Fa; HuB219; LEP R; LEPR; LEPROT; Leptin receptor fatty; Leptin receptor gene related protein; Leptin receptor precursor; OB R; OB-R; OB R gene related protein; OB receptor; OB-RGRP; Obr; Ob-R a/b/c/d/e; LEPR_HUMAN; LEPR_HUMAN; LEPRD; Leptin receptor.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 信号转导 细胞膜受体
抗体来源Mouse
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
Leptin receptor,瘦素受体抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量132/99kDa
细胞定位细胞膜 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Leptin receptor/OB-R
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Leptin receptor,瘦素受体抗体PubMedPubMed
产品介绍background:
Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.

Function:
Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway

Subunit:
On leptin stimulation, homodimerizes. The phosphorylated receptor binds a number of SH2 domain-containing proteins such as STAT3, PTPN11, and SOCS3. Interaction with SOCS3 inhibits LRb signaling.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Isoform E: Secreted (Probable).

Tissue Specificity:
Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.

Post-translational modifications:
On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3. Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.

Leptin receptor,瘦素受体抗体Similarity:
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like (immunoglobulin-like) domain.

Database links:
Entrez Gene: 3953 Human
Entrez Gene: 16847 Mouse
Entrez Gene: 24536 Rat
Omim: 601007 Human
SwissProt: P48357 Human
SwissProt: P48356 Mouse
SwissProt: Q62959 Rat
Unigene: 23581 Human
Unigene: 723178 Human
Unigene: 259282 Mouse
Unigene: 9891 Rat

Leptin receptor,瘦素受体抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Leptin是一种分子量为16kDa的脂肪组织源***，由脂肪细胞分泌的，具有强亲水性，以单体形式存在于血浆中。 Leptin 具有广泛的生物学效应，作用于下丘脑，调节食欲、能量代谢及体重。Leptin 还可能作为脂肪-胰岛内分泌轴一部分，参与胰岛素分泌的调节。Leptin receptor (OB-R)是与gp130最密切相关的信号转导的共同成分,属1类细胞因子超家族，下丘脑有丰富的Leptin receptor，在其它部位也有分布，但水平较低，如：胰岛素B细胞、脉络丛、肝、肺、心、肾、睾丸、淋巴细胞和脂肪细胞中。人类存在四个异性体，均为单跨膜受体。其中OB-Rb在胞内结构域最长，是唯一能进行信号传导并调节热量摄入和能量消耗的异性体,分子量为132kDa。