CRLR,降钙素基因相关肽1型受体抗体

产品编号HZ-1860R
英文名称CRLR
中文名称降钙素基因相关肽1型受体抗体
别 名CGRPR; calcitonin receptor like precursor; Calcitonin gene related peptide type 1 receptor; Calcitonin receptor like; CALCRL; CGRP type 1 receptor; CGRPR; Calcitonin gene related peptide type 1 receptor; Calcitonin receptor like; Calcitonin Receptor Like Receptor; CALCRL; CGRP type 1 receptor; CALRL_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 神经生物学 信号转导 G蛋白偶联受体
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
CRLR,降钙素基因相关肽1型受体抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量50kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CGRPR1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CRLR,降钙素基因相关肽1型受体抗体PubMedPubMed
产品介绍background:
CRLR is a receptor for calcitonin gene related peptide type 1. The activity of CRLR is mediated by G proteins which activate adenylyl cyclase. CRLR expression has been reported in brain, lung, blood vessel, liver, and intestinal tract. ESTs have been isolated from B Cell/lung/testis, bone marrow, embryo, lung, and synovium libraries.

Function:
Receptor for calcitonin-gene-related peptide (CGRP) together with RAMP1 and receptor for adrenomedullin together with RAMP3 (By similarity). Receptor for adrenomedullin together with RAMP2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Subunit:
Heterodimer of CALCRL and RAMP1, RAMP2 or RAMP3.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Predominantly expressed in the lung and heart.

Similarity:
Belongs to the G-protein coupled receptor 2 family.

Gene ID:
10203

CRLR,降钙素基因相关肽1型受体抗体Database links:
Entrez Gene: 10203 Human
Entrez Gene: 54598 Mouse
Entrez Gene: 397172 Pig
Entrez Gene: 25029 Rat
Entrez Gene: 527140 Cow
Omim: 114190 Human
SwissProt: Q16602 Human
SwissProt: Q9R1W5 Mouse
SwissProt: Q8WN93 Pig
SwissProt: Q63118 Rat
Unigene: 470882 Human
Unigene: 11202 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Western blot analysis of extracts from Lung lumph using CRLR antibody. (Primary dilution:1:300) Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CRLR Polyclonal Antibody, Unconjugated(HZ-1860R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining