TSKS,睾丸特异激酶底物蛋白抗体
英文名称： Anti-TSKS antibody
型号:null    抗体货号： HZ-11413R
价格:请致电：010-57128832,18610462672
品牌: 中国/美国    产品商标: hzbscience

TSKS,睾丸特异激酶底物蛋白抗体

产品编号HZ-11413R
英文名称TSKS
中文名称睾丸特异激酶底物蛋白抗体
别 名STK22 substrate 1; Stk22s1; Testis specific kinase substrate; Testis specific serine kinase substrate; TSKS; TSKS1; MGC134104; TSKS_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 发育生物学 神经生物学 信号转导 干细胞
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
TSKS,睾丸特异激酶底物蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量65kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human TSKS (121-170aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
TSKS,睾丸特异激酶底物蛋白抗体PubMedPubMed
产品介绍background:
TSKS is a 592 amino acid protein that is highly expressed in human testicular tissue. Low levels of TSKS are detectable in prostate, placenta, fetal liver, thymus, and mammary gland tissues. TSKS is found to be downregulated in cancerous testicular tissue from seminoma, teratocarcinoma, embryonal and Leydig cell tumors concurrently with high expression in neighboring premalignant carcinoma. TSKS protein contains an N-terminal signal peptide, but does not contain a transmembrane region. TSKS has many potential phosphorylation and glycosylation sites and is phosphorylated by soluble recombinant TSSK2 in vitro. It is thought that TSKS likely plays a physiological role in spermatogenesis or spermiogenesis.

Function:
A novel putative serine/threonine kinase substrate gene (TSKS, for testis-specific kinase substrate) is likely the human homolog of the mouse testis-specific serine kinase substrate gene. TSKS exhibits high levels of expression exclusively in human testicular tissue. The expression of TSKS is downregulated in cancerous testicular tissue, in comparison to adjacent normal tissue. These observations suggest a role of TSKS in testicular physiology, most probably in the process of spermatogenesis or spermiogenesis.

Subcellular Location:
Cytoplasmic

Tissue Specificity:
Highly expressed in testis. Expressed at low levels in prostate, female breast, placenta, ovary and thymus.

Post-translational modifications:
Phosphorylated on serine residue(s) by STK22A/TSSK1 and STK22B/TSSK2.

Database links:
UniProtKB/Swiss-Prot: Q9UJT2.3

TSKS,睾丸特异激酶底物蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

ked polyubiquitin chains via interaction with the ubiquitin-conjugating UBE2L6/UBCH8, leading to degradation of substrate proteins such as CHEK2, JMJD2A/KDM4A and KU80/XRCC5: it is still unclear how the preference toward 'Lys-48'- versus 'Lys-63'-linked ubiquitination is regulated but it could be due to RNF8 ability to interact with specific E2 specific ligases. For instance, interaction with phosphorylated HERC2 promotes the association between RNF8 and UBE2N/UBC13 and favors the specific formation of 'Lys-63'-linked ubiquitin chains. Promotes non-homologous end joining (NHEJ) by promoting the 'Lys-48'-linked ubiquitination and degradation the of KU80/XRCC5. Following DNA damage, mediates the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF168, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation. Involved in the activation of ATM by promoting histone H2B ubiquitination, which indirectly triggers histone H4 'Lys-16' acetylation (H4K16ac), establishing a chromatin environment that promotes efficient activation of ATM kinase. Required in the testis, where it plays a role in the replacement of histones during spermatogenesis. At uncapped telomeres, promotes the joining of deprotected chromosome ends by inducing H2A ubiquitination and TP53BP1 recruitment, suggesting that it may enhance cancer development by aggraving telomere-induced genome instability in case of telomeric crisis. Promotes the assembly of RAD51 at DNA DSBs in the absence of BRCA1 and TP53BP1 Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2.

RNF8,环指蛋白8抗体Subunit:
Homodimer. Forms a E2-E3 ubiquitin ligase complex composed of the RNF8 homodimer and a E2 heterodimer of UBE2N and UBE2V2. Interacts with class III E2s, including UBE2E1, UBE2E2, and UBE2E3 and with UBE2N. Interacts with RXRA. Interacts (via FHA domain) with ATM-phosphorylated MDC1. Interacts (via FHA domain) with 'Thr-4827' phosphorylated HERC2 (via C-terminus). Interacts (via FHA domain) with phosphorylated human herpesvirus 1 ICP0 protein; leading to RNF8 degradation by the proteasome.

Subcellular Location:
Nucleus. Midbody. Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be associated with condensed chromosomes at any time during the cell cycle.

Tissue Specificity:
Ubiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells.

Post-translational modifications:
Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin. 'Lys-63' polyubiquitination is mediated by UBE2N. 'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger.

Similarity:
Belongs to the RNF8 family.
Contains 1 FHA domain.
Contains 1 RING-type zinc finger.

Gene ID:
9025

Database links:
Entrez Gene: 9025 Human
Omim: 611685 Human
SwissProt: O76064 Human
Unigene: 485278 Human

RNF8,环指蛋白8抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.