Caspase 14,半胱胺酸蛋白酶蛋白14抗体

产品编号HZ-10136R
英文名称Caspase 14
中文名称半胱胺酸蛋白酶蛋白14抗体
别 名Apoptosis related cysteine protease; Apoptosis related cysteine protease; CASP 14; CASP-14; CASP14; Caspase 14 apoptosis related cysteine protease; Caspase 14 apoptosis related cysteine protease; Caspase 14 precursor; Caspase 14 precursor; Caspase-14 subunit p10; Caspase-14; Caspase-14; Caspase14; CASPE_HUMAN; MGC119079; MICE; MICE; Mini ICE; Mini ICE.
说 明 书0.1ml 0.2ml
研究领域细胞生物 细胞凋亡
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Guinea Pig,
Caspase 14,半胱胺酸蛋白酶蛋白14抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量27kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Caspase 14
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Caspase 14,半胱胺酸蛋白酶蛋白14抗体PubMedPubMed
产品介绍background:
A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is composed of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6 and caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9, caspase-10, and caspase-14. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-14, also designated MICE (for mini-ICE), is highly expressed in embryonic tissues but appears to be absent from adult tissues. Procaspase-14 can be processed in vitro by caspase-8 and caspase-10 but not by other caspases.

Function:
Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin.

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels.

Similarity:
Belongs to the peptidase C14A family.

Gene ID:
23581

Caspase 14,半胱胺酸蛋白酶蛋白14抗体Database links:
Entrez Gene: 23581 Human
Entrez Gene: 12365 Mouse
Entrez Gene: 299587 Rat
Omim: 605848 Human
SwissProt: P31944 Human
SwissProt: O89094 Mouse
Unigene: 466057 Human
Unigene: 20940 Mouse
Unigene: 228179 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-caspase-14 Polyclonal Antibody, Unconjugated(HZ-10136R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Caspase-14 Polyclonal Antibody, Unconjugated(HZ-10136R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Mouse Embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Caspase 14 Polyclonal Antibody, Unconjugated(HZ-10136R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining