Catalog Number:  26921

Gene Symbol:  RAB4

Description:   Anti-Active Rab4 Mouse Monoclonal Antibody

Background:   Rab-4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, SGSM1, SGSM2 and SGSM3. It participates in protein transport and in vesicular traffic.

Immunogen:   Recombinant full length protein of active Rab4A.

Applications:   IP, IHC

Recommended Dilutions:   1 μg for 1~2 mg total cellular proteins for IP 1:50~100 dilution for IHC

Concentration:   1 mg/ml

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Anti-active Rab4 antibody recognizes active Rab4 from vertebrates.

Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

Rab4A activation assay. Purified GST-tagged Rab4A proteins (Cat. #10130) were immunoprecipitated with the anti-active Rab4 monoclonal antibody (Cat. No. 26921) after treated with GDP (lane 1) or GTPγ S(lane 2), and was blotted with anti-Rab4A polyclonal antibody (Cat. No. 21067). Input control is shown in the bottom panel.

Publications:

1.  VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors     Hepatology Volume 61, Issue 1, pages 361–374, January 2015

2.  Rab5 Activity Regulates GLUT4 Sorting into ***-Responsive and Non-***-Responsive Endosomal Compartments: A Potential Mechanism for Development of *** Resistance     Endocrinology. 2014 Sep;155(9):3315-28