Anti-Active Rab35 Mouse Monoclonal Antibody
英文名称: Anti-Active Rab35 Mouse Monoclonal Antibody
型号:null    抗体货号: 26922
价格:请致电:010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

Catalog Number:  26922
Gene Symbol:  RAB35
Description:   Anti-Active Rab35 Mouse Monoclonal Antibody
Background:   The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions.
Immunogen:   Recombinant full length protein of active Rab35
Applications:   IP, IHC, IF
Recommended Dilutions:   
1 µg for ~2 mg total cellular proteins for IP
1:100 dilution for IHC, IF
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Rab35 antibody recognizes active Rab35 proteins of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Immunoprecipitation/Western blot:  
Rab35 activation assay. Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-active Rab35 monoclonal antibody (Cat. No. 26922). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. No. 21078).
 
Publications:
1.  VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors
    Hepatology Volume 61, Issue 1, pages 361–374, January 2015
2.  Rab5 Activity Regulates GLUT4 Sorting into ***-Responsive and Non-***-Responsive Endosomal Compartments: A Potential Mechanism for Development of *** Resistance
    Endocrinology. 2014 Sep;155(9):3315-28