Anti-Active Ran Mouse Monoclonal Antibody
英文名称： Anti-Active Ran Mouse Monoclonal Antibody
型号:null    抗体货号： 26915
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

Active Ran-GTP [26915]
[30 µL]		  

Catalog Number:  26915
Gene Symbol:  RAN
Description:   Anti-Active Ran Mouse Monoclonal Antibody
Background:   Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is invovled in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis.
Immunogen:   Recombinant full length protein of active Ran.
Applications:   IP, IHC
Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP;
1:100 dilution for IHC
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Ran antibody recognizes active Ran of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Immunoprecipitation/Western blot:  
Ran activation assay. Purified Ran proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Ran monoclonal antibody (Cat. No. 26915). Immunoblot was with an anti-Ran polyclonal antibody.
 

 

Publications:
1.  NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3
    Nucleic Acids Res. 2012 Sep 1;40(17):8484-98