COX4,细胞色素c氧化酶IV亚型1抗体
英文名称: Anti-COX4 antibody
型号:null    抗体货号: HZ-1533R
价格:请致电:010-57128832,18610462672
品牌: 中国/美国    产品商标: hzbscience

COX4,细胞色素c氧化酶IV亚型1抗体

产品编号HZ-1533R
英文名称COX4
中文名称细胞色素c氧化酶IV亚型1抗体
别 名COX IV; COX 4; COX 4I1; COX 4I2; COXIV Isoform 2; COX IV 1; COX IV-1; COX IV; COX4 2; COX4; COX4B; COX4I1; COX4I2; COX4L2; COXIV 2; COXIV; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome C Oxidase Subunit IV; Cytochrome c oxidase subunit IV isoform 1; Cytochrome c oxidase subunit IV isoform 2 (lung); dJ857M17.2; MGC72016; COX IV-1; Cox4a Cytochrome c oxidase polypeptide IV; COX41_HUMAN; COX4I1.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Cow,
COX4,细胞色素c氧化酶IV亚型1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-200 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量17kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human COX IV-1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
COX4,细胞色素c氧化酶IV亚型1抗体PubMedPubMed
产品介绍background:
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008]

Function:
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Subcellular Location:
Mitochondrion inner membrane.

Tissue Specificity:
Ubiquitous.

Similarity:
Belongs to the cytochrome c oxidase IV family.

Gene ID:
1327

COX4,细胞色素c氧化酶IV亚型1抗体Database links:
Entrez Gene: 1327 Human
Entrez Gene: 12857 Mouse
Entrez Gene: 29445 Rat
Entrez Gene: 281090 Cow
Omim: 123864 Human
SwissProt: P00423 Cow
SwissProt: Q3SZS0 Cow
SwissProt: P13073 Human
SwissProt: P19783 Mouse
SwissProt: P10888 Rat
Unigene: 433419 Human
Unigene: 386758 Mouse
Unigene: 2528 Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

细胞色素c氧化酶4亚型1抗体;(细胞色素c氧化酶IV亚型1)是一种存在线粒体上的大型跨膜蛋白复合物。由于细胞色素氧化酶是呼吸电子传递链的第四个中心酶复合物,因此又被称为复合物IV。

> Rac activation assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti-active Rac monoclonal antibody (Cat # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti-Rac rabbit polyclonal antibody (Cat # 21003). The bottom panel shows the Western blot with anti-Rac of the cell lysates used (5% of that used in the top panel).
Immunofluorescence:  
Immunofluorescence for the active Rac using Anti-Active Rac1-GTP Mouse Monoclonal Antibody [26903] shows Rac-GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections.
The tissue sections were fixed with -20 ºC methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rabbit AlexaFluor594 at 1:1000.
 

 

Publications:
1.  Phosphorylation of α-tubulin by protein kinase C stimulates microtubule dynamics in human breast cells
Cytoskeleton Volume 71, Issue 4, pages 257–272, April 2014
2.  Immunofluorescence and Confocal Microscopy of Neutrophils
Neutrophil Methods and Protocols Methods in Molecular Biology Volume 1124, 2014, pp 251-268
3.  A link between the nuclear-localized srGAP3 and the SWI/SNF chromatin remodeler Brg1
Molecular and Cellular Neuroscience Volume 60, May 2014, Pages 10–25
4.  SHP2 Phosphatase Promotes Mast Cell Chemotaxis toward Stem Cell Factor via Enhancing Activation of the Lyn/Vav/Rac Signaling Axis
The Journal of Immunology April 14, 2014 1301155
5.  ROBO1, a tumor suppressor and critical molecular barrier for localized tumor cells to acquire invasive phenotype: Study in African-American and Caucasian prostate cancer models
International Journal of Cancer Volume 135, Issue 11, pages 2493–2506, 1 December 2014
6.  Transcription factors NRF2 and nf-kb are coordinated effectors of the RHO family, GTP binding protein rac1 during inflammation
J Biol Chem. 2014 May 30;289(22):15244-58
7.  Canonical and Non-canonical G-protein Signaling Helps Coordinate Actin Dynamics to Promote Macrophage Phagocytosis of Zymosan
Mol Cell Biol. 2014 Nov 15;34(22):4186-99
8.  p53 Down Regulates PDGF-Induced Formation of Circular Dorsal Ruffles in Rat Aortic Smooth Muscle Cells
PLoS One. 2014 Sep 23;9(9):e108257
9.  LPA-mediated migration of ovarian cancer cells involves translocalization of Gαi2 to invadopodia and association with Src and β-pix
Cancer Lett. 2015 Jan 28;356(2 Pt B):382-91
10.  cAMP controls the restoration of endothelial barrier function after thrombin-induced hyperpermeability via Rac1 activation
Physiological ReportsPublished 24 October 2014Vol. 2no. e12175DOI: 10.14814/phy2.12175
11.  Cisplatin at Sub-toxic Levels Mediates Integrin Switch in Lung Cancer Cells
Anticancer Research December 2014 vol. 34 no. 12 7111-7117
12.  Semaphorin-3F suppresses the stemness of colorectal cancer cells by inactivating Rac1
Cancer letters. March 1, 2015Volume 358, Issue 1, Pages 76–84.
13.  Geometry sensing through POR1 regulates Rac1 activity controlling early osteoblast differentiation in response to nanofiber diameter
Integr Biol (Camb). 2014 Dec 24
14.  Inhibition of Rac1 Activity in the Hippocampus Impairs the Forgetting of Contextual Fear Memory
Mol Neurobiol. 2015 Jan 24