Catalog Number:  26916

Gene Symbol:  SAR1

Description:   Anti-Active Sar1 Mouse Monoclonal Antibody

Background:   Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER.

Immunogen:   Recombinant full length protein of active Sar1a.

Applications:   IP, IHC

Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC

Concentration:   1 mg/ml

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Anti-active Sar1 antibody recognizes active Sar1 of vertebrates.

Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

Sar1 activation assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti-active Sar1 monoclonal antibody (Cat. No. 26916). Immunoblot was with an anti-Sar1 rabbit polyclonal antibody (Cat. No. 21090).