EGF,小鼠抗表皮生长因子抗体
英文名称: Anti-EGF antibody
型号:null    抗体货号: HZ-4568M
价格:请致电:010-57128832,18610462672
品牌: 中国/美国    产品商标: hzbscience

EGF,小鼠抗表皮生长因子抗体

产品编号HZ-4568M
英文名称EGF
中文名称小鼠抗表皮生长因子抗体
别 名Beta urogastrone; EGF; Egf; EGF_HUMAN; Epidermal Growth Factor; Epidermal Growth Factor; HOMG4; OTTHUMP00000219721; OTTHUMP00000219722; Pro epidermal growth factor; URG; Urogastrone.
说 明 书0.1ml 0.2ml
研究领域肿瘤 信号转导 生长因子和***
抗体来源Mouse
克隆类型Polyclonal
交叉反应 Mouse, Rat,
EGF,小鼠抗表皮生长因子抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 IEM=1:20-200 IGS=1:20-200 GICA=1:20-200 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量5.8/133kDakDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from mouse EGF
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
EGF,小鼠抗表皮生长因子抗体PubMedPubMed
产品介绍background:
Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid protein originally found in rodents and humans. It has been shown to be a potent mitogen for a variety of cell types both in vivo and in vitro. EGF binds to the EGF receptor on the surface of cells and mediates intrinsic phosphorylation of the receptor on tyrosine residues. It has been detected in nearly all body fluids, such as urine (urogastrone), saliva, milk and platelet-rich plasma. EGF, TGF?and vaccinia virus growth factor exhibit 30-40% amino acid homology. Several additional members of the EGF/TGF family have been described; these include Cripto, Amphiregulin and the heparin-binding EGF-like growth factor. Amphiregulin and the heparin-binding EGF-like growth factor both bind to the EGF receptor.

Function:
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.

Subunit:
Interacts with EGFR and promotes EGFR dimerization. Interacts with RHBDF2. Interacts with RHBDF1; may retain EGF in the endoplasmic reticulum and regulates its degradation through the endoplasmic reticulum-associated degradation (ERAD).

EGF,小鼠抗表皮生长因子抗体Subcellular Location:
Membrane.

Tissue Specificity:
Expressed in kidney, salivary gland, cerebrum and prostate.

Post-translational modifications:
Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.

DISEASE:
Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.

Similarity:
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.

Gene ID:
1950

Database links:
Entrez Gene: 13645 Mouse
Entrez Gene: 1950 Human
Omim: 131530 Human
SwissProt: P01133 Human
SwissProt: P01132 Mouse
Unigene: 419815 Human
Unigene: 252481 Mouse

EGF,小鼠抗表皮生长因子抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.