EST1A,端粒酶结合蛋白EST1A抗体

产品编号HZ-20031R
英文名称EST1A
中文名称端粒酶结合蛋白EST1A抗体
别 名C17orf31; EST1-like protein A; Ever shorter telomeres 1A; hSmg5/7a; KIAA0732; Smg-6 homolog; SMG6; Telomerase subunit EST1A; Telomerase-binding protein EST1A; EST1A_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Sheep,
EST1A,端粒酶结合蛋白EST1A抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量160kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human EST1A
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
EST1A,端粒酶结合蛋白EST1A抗体PubMedPubMed
产品介绍background:
This gene encodes a component of the telomerase ribonucleoprotein complex responsible for the replication and maintenance of chromosome ends. The encoded protein also plays a role in the nonsense-mediated mRNA decay (NMD) pathway, providing the endonuclease activity near the premature translation termination codon that is needed to initiate NMD. Alternatively spliced transcript variants encoding distinct protein isoforms have been described. [provided by RefSeq, Feb 2014]

Function:
Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. May have a general role in telomere regulation. Promotes in vitro the ability of TERT to elongate telomeres. Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization. Binds to the single-stranded 5'-(GTGTGG)(4)GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER).
Plays a role in nonsense-mediated mRNA decay. Is thought to provide a link to the mRNA degradation machinery as it has endonuclease activity required to initiate NMD, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA.

Subunit:
Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC). Interacts with TERT, independently of the telomerase RNA. Interacts with PP2A catalytic subunits, SMG1, UPF1, UPF2 and UPF3B.

Subcellular Location:
Nucleus, nucleolus. Chromosome, telomere (Probable). Cytoplasm, cytosol. Note=Particularly enriched in the nucleolus.

EST1A,端粒酶结合蛋白EST1A抗体Tissue Specificity:
Ubiquitous.

Post-translational modifications:

DISEASE:

Similarity:
Contains 1 PINc domain.

Gene ID:
23293

Database links:
Entrez Gene: 23293 Human
SwissProt: Q86US8 Human
SwissProt: P61406 Mouse
Unigene: 448342 Human

EST1A,端粒酶结合蛋白EST1A抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.