Epac2,鸟嘌呤核苷酸交换因子4抗体

产品编号HZ-10955R
英文名称Epac2
中文名称鸟嘌呤核苷酸交换因子4
别 名CAMP GEFII; cAMP regulated guanine nucleotide exchange factor II; cAMP-GEFII; cAMP-regulated guanine nucleotide exchange factor II; CAMPGEFII; CGEF 2; CGEF2; Epac 2; EPAC2; Exchange factor directly activated by cAMP 2; Exchange protein directly activated by cAMP 2; Nbla00496; Putative protein product of Nbla00496; Rap guanine nucleotide exchange factor (GEF) 4; RAP guanine nucleotide exchange factor 4; RAPGEF 4; RAPGEF4; RPGF4_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
Epac2,鸟嘌呤核苷酸交换因子4抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量116kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Epac2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Epac2,鸟嘌呤核苷酸交换因子4抗体PubMedPubMed
产品介绍background:
3',5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac1 (cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the ras family GTPases Rap1 and Rap2 by promoting GTP binding in a cAMP-dependent manner (1,2). Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission (3). Human EPAC2 contains at least 31 exons and maps to chromosome 2q31 (4). The 4.4-kb Epac2 transcript is prominent in brain and adrenal gland (5,6). Within the brain, expression is strong in cortex, occipital pole, frontal lobe, temporal lobe, amygdala, putamen, hippocampus and cerebellum (5,6).

Function:
Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.

Subunit:
Interacts with RIMS1 and RIMS2. Probably part of a complex with RIMS2 and GTP-activated RAB3A.

Subcellular Location:
Cytoplasm. Membrane.

Epac2,鸟嘌呤核苷酸交换因子4抗体Tissue Specificity:
Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.

Similarity:
Contains 2 cyclic nucleotide-binding domains. Contains 1 DEP domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain.

Gene ID:
11069

Database links:
Entrez Gene: 11069 Human
Entrez Gene: 56508 Mouse
Entrez Gene: 252857 Rat
Omim: 606058 Human
SwissProt: Q8WZA2 Human
SwissProt: Q9EQZ6 Mouse
SwissProt: Q9Z1C7 Rat
Unigene: 470646 Human
Unigene: 196153 Mouse
Unigene: 42890 Rat

Epac2,鸟嘌呤核苷酸交换因子4抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Protein: line1,mouse liver lysate;line2,mouse brain lysate; Primary: rabbit Anti-Epac2 (HZ-10955R) at 1:300;  Secondary: HRP conjugated Goat-Anti-rabbit IgG(HZ-0295G-HRP) at 1: 5000;  Predicted band size: 116 kD Observed band size: 116 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Epac2 Polyclonal Antibody, Unconjugated(HZ-10955R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining