PIK3CA (D549N) [26215]
[100ul] | |
| Catalog Number: 26215 | | Gene Symbol: PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha | | Description: Anti-PIK3CA (D549N) Mouse Monoclonal Antibody | | Background: Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer. | | Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of D549N, human origin. | | Applications: ELISA, WB, IHC | Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100 | | Concentration: 0.5 mg/ml | | Host: Mouse | | Format: Liquid | | Clonality: Monoclonal | | Isotype: | | Purity: Purified from ascites | | Storage buffer: | | Preservative: no | | Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | | Species Reactivity: Recognizes D549N mutant, but not wild-type PIK3CA of vertebrates. | | Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | | | | Western blot: |  | Western blot analysis of recombinant PIK3CA (D549N) and wildtype proteins. Purified His-tagged PIK3CA (D549N) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-PIK3CA (D549N) monoclonal antibody (Cat. #26215). |  | Immunofluorescence of cells expressing PIK3CA proteins with anti-PIK3CA (D549N) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA (D549N) plasmid (right column), then fixed and stained with anti-PIK3CA (D549N) monoclonal antibody (Cat. #26215). | | |