Pyruvate Assay Kit
英文名称: Pyruvate Assay Kit
型号:null    抗体货号: ab65342
价格:请致电:010-57128832,18610462672
品牌: 英国    产品商标: abcam

Overview

Product name

Pyruvate Assay Kit

Product overview

The Pyruvate Assay Kit provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc. In the assay, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate colour and fluorescence. The colour or fluorescence intensity is proportional to pyruvate content. Thus the pyruvate concentrations can be accurately measured by either colorimetric (spectrophotometry at OD = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The kit detects 1-1000 µM pyruvate samples.

Notes

Pyruvate quickly used up in different metabolic reactions so we recommend using samples as soon as they are harvested or extracted. We also recommend to deproteinate samples to remove interfering enzymes using either PCA or our 10 kDa spin cut-off filters (ab93349) after which they can be aliquoted in working volumes and stored at -80C until further use.

Tested applications

Functional Studiesmore details

Properties

Storage instructions

Store at -20°C, Stable for 6 months at -20°C

Components Identifier 100 tests
DMSO (anhydrous) Brown 1 x 400µl
Pyruvate Assay Buffer WM 1 x 25ml
Pyruvate Enzyme Mix (Lyophilized) Green 1 vial
Pyruvate Probe (Lyophilized) Red 1 vial
Pyruvate Standard (100 nmol/µl) Yellow 1 x 100µl

http://www.abcam.com/pyruvate-assay-kit-ab65342.html

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