Overview
Product name
Choline/Acetylcholine Assay Kit
Product overview
The Choline/Acetylcholine Assay Kit provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric (spectrophotometry at OD = 570 nm) or fluorometric (Ex/Em = 535/587 nm) method. In the assay free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate colour, and fluorescence. Acetylcholine can be converted to choline by adding acetylcholinesterase to the reaction. The kit can detect choline and acetylcholine (total choline – free choline) in various biological samples such as in blood, cells, culture media, fermentation media, etc. There is no need for pretreatment or purification of samples. The kit can detect 10 pmol~5 nmol of choline or acetylcholine.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at -20°C, Stable for 6 months at -20°C
Storage buffer
Preservative: None
 
    
        
            
Components
            
Identifier
            
100 tests
        
    
    
        
            
Acetylcholinesterase
            
Blue
            
1 vial
        
        
            
Choline Assay Buffer
            
WM
            
1 x 25ml
        
        
            
Choline Enzyme Mix
            
Green
            
1 vial
        
        
            
Choline Probe (in DMSO)
            
Red
            
1 x 200µl
        
        
            
Choline Standard (5 µmol)
            
Yellow
            
1 vial
        
    
http://www.abcam.com/Choline-Acetylcholine-Assay-Kit-ab65345.html