Overview
Product name
Cholesterol/Cholesteryl Ester Quantitation Kit
Product overview
The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of cholesterol, cholesteryl ester, or both by either colorimetric or fluorometric methods. A large portion of the cholesterol in blood is in the form of cholesteryl esters. Cholesterol esterase hydrolizes cholesteryl ester into cholesterol. Cholesterol is then oxidized by cholesterol oxidase to yield H2O2. The produced H2O2 interacts with a sensitive cholesterol probe to produce resorufin, which can be detected by spectrophotometry at OD = 570 nm or fluorometry at Ex/Em = 535/587 nm. The assay can detect cholesterol itself (without adding cholesterol esterase) or total cholesterol (cholesterol + cholesteryl ester) by adding cholesterol esterase to the reaction, or cholesteryl ester itself by subtracting the value of cholesterol from the total value of cholesterol and cholesteryl esters.
Notes
Store kit at –20oC, protect from light. Allow reagents to warm to room temperature and briefly centrifuge vials before opening.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
    
        
            
Components
            
Identifier
            
100 tests
        
    
    
        
            
Cholesterol Assay Buffer
            
WM
            
1 x 25ml
        
        
            
Cholesterol Esterase (lyophilized)
            
Blue
            
1 vial
        
        
            
Cholesterol Probe (in DMSO, anhydrous)
            
Red
            
1 x 200µl
        
        
            
Cholesterol Standard (2 µg/µl )
            
Yellow
            
1 x 100µl
        
        
            
Enzyme Mix (lyophilized)
            
Green
            
1 vial
        
    
http://www.abcam.com/Cholesterol-Cholesteryl-Ester-Quantitation-Kit-ab65359.html