Overview
Product name
cGMP Direct Immunoassay Kit
Product overview
The cGMP Direct Immunoassay Kit provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples. The kit utilizes the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cGMP in samples. The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04-10 pmol/50 µl cGMP samples.
Notes
The component in Vial B is sensitive to evaporation. The vial should be tightly capped always and has to be kept only at 4C and not left at room temperature.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at -20°C, Stable for 6 months at -20°C
Storage buffer
Preservative: None
Constituents: BSA
    
        
            
Components
            
Identifier
            
100 tests
        
    
    
        
            
10X cGMP Assay Buffer
            
WM
            
1 x 25ml
        
        
            
Acetylating Reagent A
            
Violet
            
1 x 750µl
        
        
            
Acetylating Reagent B
            
Brown
            
1 x 1.5ml
        
        
            
Anti-cGMP pAb/BSA
            
Red
            
1 vial
        
        
            
cGMP-HRP/BSA
            
Green
            
1 vial
        
        
            
HRP Developer
            
Amber
            
1 x 10ml
        
        
            
Neutralizing Buffer
            
NM
            
1 x 7.5ml
        
        
            
Standard cGMP (10 nmol)
            
Yellow
            
1 vial
        
    
http://www.abcam.com/cGMP-Direct-Immunoassay-Kit-ab65356.html