Overview
Product overview
The Plasma Phospholipid Transfer Protein Drug Screening Kit utilizes rabbit serum as Plasma Phospholipid Transfer Protein activity source to screen for Plasma Phospholipid Transfer Protein inhibitors. The assay uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of Plasma Phospholipid Transfer Protein. Plasma Phospholipid Transfer Protein mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Excitation: 465 nm; Emission: 535 nm). An inhibitor of Plasma Phospholipid Transfer Protein will inhibit the lipid transfer and therefore decrease fluorescence intensity. The kit provides sufficient reagents for 100 Plasma Phospholipid Transfer Protein inhibitor screening assays.
Properties
Storage instructions
Store at +4°C.
Components | Identifier | 100 tests |
PLTP Acceptor Molecule | Blue | 1 x 1ml |
PLTP Assay Buffer (10X) | Clear | 1 x 5ml |
PLTP Donor Molecule | Violet | 1 x 1ml |
Positive Control (Rabbit Serum) | Green | 1 x 300µl |
http://www.abcam.com/PLTP-Inhibitor-Drug-Screening-Kit-ab65387.html