Jackson Immunoresearch免疫封闭血清 英文名称: Jackson Immunoresearch免疫封闭血清 | ||||||||||||||||||||||||||||||||||||||||||||||||
型号:null 抗体货号: | ||||||||||||||||||||||||||||||||||||||||||||||||
价格:请致电:010-57128832,18610462672 | ||||||||||||||||||||||||||||||||||||||||||||||||
品牌: 美国 产品商标: jackson | ||||||||||||||||||||||||||||||||||||||||||||||||
1 Jackson二抗产品 Anti- Horse IgG 抗马IgG二抗 Anti- Hamster IgG 抗仓鼠IgG二抗 Anti- Dog IgG 抗狗IgG二抗 Anti- Chicken IgG 抗鸡IgG二抗 Anti- Cat IgG 抗猫IgG二抗 Anti- Guinea Pig IgG 抗豚鼠IgG二抗 Anti-Sheep IgG 抗绵羊IgG二抗 Anti-Goat IgG 抗山羊IgG二抗 Anti-Rabbit IgG 抗兔IgG二抗 Anti-Human IgG 抗人IgG二抗 Anti-Rat IgG 抗大鼠IgG二抗 Anti-Mouse IgG Subclass Specific*二抗 Anti-Mouse IgG的二抗 抗小鼠IgG二抗 Anti-Bovine IgG的二抗 抗牛IgG二抗 以上二抗均有未标记的,标记好DyLight405,DyLight594, DyLight649,CoumarinAMCA, CyanineCy2, CyanineCy5,DyLight488, Fluorescein FITC生物素 Biotin-SP(long spacer),DyLight549, Cyanine Cy3, Rhodamine TRITC, Texas Red TR,Horseradish Peroxidase(HRP标记),Alkaline Phosphatase(碱性***酸酶标记)的二抗,详情请咨询我公司。或查询http://www.jacksonimmuno.com/ 2 封闭血清 BSA和奶粉可能含有IgG与以上抗体发生较强的反应。因此,使用BSA或奶粉封闭或稀释抗体会增加背景或降低抗体效价。此种情况下推荐二抗物种的正常血清(5% v/v)用于封闭。
Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze-dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking agent to reduce background from non-specific, conserved-sequence, and/or Fc-receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody. Gamma globulins are further purified from non-immunized animal serums by salt fractionation, ion-exchange chromatography, and gel filtration. Gamma globulins are an inexpensive source of IgG with only trace amounts of other immunoglobulins and/or non-immunoglobulin serum proteins. Gamma globulins are supplied as sterile liquids in phosphate buffer without stabilizers or preservatives |