Anti-HRAS (G13A) Mouse Monoclonal Antibody
英文名称： Anti-HRAS (G13A) Mouse Monoclonal Antibody
型号:null    抗体货号： 26191
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

Ras (G13A) [26191]
[100ul]		  

Catalog Number:  26191
Gene Symbol:  H-Ras; K-Ras; N-Ras
Description:   Anti-HRAS (G13A) Mouse Monoclonal Antibody
Background:   Belongs to the small GTPase superfamily. Ras family binds GDP/GTP and possess intrinsic GTPase activity, alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).Located in cell membrane, lipid-anchor, cytoplasmic side and Golgi apparatus membrane.
Immunogen:   A synthetic peptide from the internal region of HRas which includes the mutation of G13A, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes G13A mutant, but not wild-type H-RAS of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant HRas (G13A) and wildtype proteins. Purified His-tagged HRas (G13A) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-HRas (G13A) monoclonal antibody (Cat. #26191).
Immunofluorescence:   
Immunofluorescence of cells expressing Ras proteins with anti-HRas (G13A) antibody. HEK293T cells were transfected with pCDNA3-GFP-HRas (WT) plasmid (left column) or pCDNA3-GFP-HRas (G13A) plasmid (right column), then fixed and stained with anti-HRas (G13A) monoclonal antibody (Cat. #26191).

 

Publications:
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
    Gene Therapy 21, 888-896 (October 2014)
2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a