Background: Anaplastic lymphoma kinase (ALK) also known as CD246 (cluster of differentiation 246) is a tyrosine kinase receptor that plays an important role in the development of the brain and nervous systems. Deficiencies in ALK, which most commonly as a fusion with other genes, are indicated in cancers.
Immunogen: A synthetic peptide from the internal region of ALK which includes the mutation of D1225N, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes D1225N mutant, but not wild-type ALK of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of recombinant ALK (D1225N) and wildtype proteins. Purified His-tagged ALK (D1225N) protein (1060-1620aa, 68KD, lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-ALK (D1225N) monoclonal antibody (Cat. #26290).