Anti-RAS (A146T) Mouse Monoclonal Antibody
英文名称：  Anti-RAS (A146T) Mouse Monoclonal Antibody
型号:null    抗体货号： 26261
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

Ras (A146T) [26261]
[100ul]		  

Catalog Number:  26261
Gene Symbol:  H-Ras; K-Ras; N-Ras
Description:   Anti-RAS (A146T) Mouse Monoclonal Antibody
Background:   The A146T mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146T mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Immunogen:   A synthetic peptide from the internal region of Ras which includes the mutation of A146T, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes A146T mutant, but not wild-type RAS of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant HRas (A146T) and wild-type proteins. Purified His-tagged Ras (A146T) protein (lane 2) and corresponding wild-type protein (lane 1) were blotted with anti-Ras (A146T) monoclonal antibody (Cat. #26261).
Immunofluorescence:  
Immunofluorescence of cells expressing HRas proteins with anti-HRas (A146T) antibody. HEK293T cells were transfected with pCDNA3-GFP-HRas (WT) plasmid (left column) or pCDNA3-GFP-HRas (A146T) plasmid (right column), then fixed and stained with anti-HRas (A146T) monoclonal antibody (Cat. #26261).

 

Publications:
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
    Gene Therapy 21, 888-896 (October 2014)
2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
    J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136