Anti-RET (C634Y) Mouse Monoclonal Antibody
英文名称：  Anti-RET (C634Y) Mouse Monoclonal Antibody
型号:null    抗体货号： 26146
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

RET (C634Y) [26146]
[100ul]		  

Catalog Number:  26146
Gene Symbol:  RET, PTC, CDHF12, CAHF16, HSCR1, MEN2A, MEN2B
Description:   Anti-RET (C634Y) Mouse Monoclonal Antibody
Background:   RET protein is encoded by the RET proto- oncogene. It is a receptor tyrosine kinase. RET loss of function mutations are associated with the development of Hirschsprung’s disease, while gain of function mutations are associated with development of various types of human cancer, including medullary thyroid carcinoma, multiple endocrine neoplasias type 2A and 2B, pheochromocytoma and parathyroid hyperplasia.
Immunogen:   A synthetic peptide from the internal region of RET which includes the mutation of C634Y, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration:   0.5 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes C634Y mutant, but not wild-type RET of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant RET (C634Y) and wildtype proteins. Purified His-tagged RET (C634Y) protein (lane1) and corresponding wild-type protein (lane2) were blotted with anti-RET (C634Y) monoclonal antibody (Cat. #26146).
Immunoprecipitation/Western blot:  

 

Immunofluorescence of cells expressing RET proteins with anti-RET (C634Y) antibody. HEK293T cells were transfected with pCDNA3-GFP-RET (WT) plasmid (left column) or pCDNA3-GFP-RET (C634Y) plasmid (right column), then fixed and stained with anti-RET (C634Y) monoclonal antibody (Cat. #26146).