Background: Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13R mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G13R, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes G13R mutant, but not wild-type RAS of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of recombinant HRas (G13R) and wildtype proteins. Purified His-tagged Ras (G13R) protein (lane 1) and corresponding wildtype protein (lane 2) were blotted with anti-Ras (G13R) monoclonal antibody (Cat. #26474).
Immunofluorescence:
Immunofluorescence of cells expressing Ras proteins with anti-Ras (G13R) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras (G13R) plasmid ,pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (G13S) plasmid , pCDNA3-GFP-Ras (G13C) plasmid , pCDNA3-GFP-Ras (G13V) plasmid , pCDNA3-GFP-Ras (G13A) plasmid , or pCDNA3-GFP-Ras (G13D) plasmid , then fixed and stained with anti-Ras (G13R) monoclonal antibody (Cat. #26474
