Anti-Ras (Q61R) Mouse Monoclonal Antibody
英文名称： Anti-Ras (Q61R) Mouse Monoclonal Antibody
型号:null    抗体货号： 26192
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

Ras (Q61R) [26192]
[100 µL]		  

Catalog Number:  26192
Gene Symbol:  RAS (Q61R)
Description:   Anti-Ras (Q61R) Mouse Monoclonal Antibody
Background:   Ras protein is a membrane associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras Q61R mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
Immunogen:    A synthetic peptide from the internal region of Ras which includes the mutation of Q61R, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA 1:100-1:5000
WB 1:100-1:1000
IHC 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes Ras (Q61R), but not wild type Ras protein from vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant Ras WT and Q61R proteins. Equal amount of purified Ras WT and Q61R proteins were loaded and blotted with anti-Ras Q61R mouse monoclonal antibody (Cat. # 26192).
Publications:
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
    Gene Therapy 21, 888-896 (October 2014)
2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
    J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136