Catalog Number:  26071

Gene Symbol:  RIC8A

Description:   Anti-Ric-8A Mouse Monoclonal Antibody

Background:   Ric-8A (Resistance to Inhibitors of Cholinesterase-8A) interacts with Gαo, Gαi, Gαq and Gα13, but not Gαs, proteins. Mammalian Ric-8A protein is a guanine nucleotide exchange factor for Gαq, Gαi, Gαo, Gα12, Gα13, but not for Gαs. C. elegans Ric8 (also named Synembryn) is required for proper asymmetric division of 1-cell-stage embryos. Ric8 is required for the generation of substantial pulling forces on astral microtubules.

Immunogen:   A synthetic peptide near the C-terminus of Ric-8A, human origin.

Applications:   ELISA, WB, IHC, IP

Recommended Dilutions:    ELISA: 1:100-1:5000 WB: 1:100-1:2000 IHC: 1:50-1:100

Concentration:   1 mg/ml

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Ric-8A antibody recognizes Ric-8A from vertebrates.

Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of Ric-8A proteins. Whole-cell lysate of HeLa cells was blotted with anti-Ric-8A monoclonal antibody (Cat. #26071).

Publications:

1.  Atypical Protein Kinase Cλ is Critical for Growth Factor Receptor-Induced Dorsal Ruffle Turnover and Cell Migration     J Biol Chem. 2013 Nov 15;288(46):32827-36