庆大霉素（GEN）酶联免疫试剂盒
英文名称： Gentamicin, GEN
型号:null    产品货号： WSK-E12A
价格:请致电：010-57128832,18610462672
品牌: cn

 【预期应用】ELISA法定性（定量）检测牛奶、组织（猪肉、猪肝、鸡肉）等样本中庆大霉素的残留量。

【概述】
庆大霉素(Gentamicin, GEN)属于氨基糖甙类抗生素，主要作用于革兰氏阴性细菌，由于其抗菌谱广、疗效佳且价格低廉而广泛应用于兽医临床和动物饲料添加剂。在其应用过程中易产生毒副作用，尤其大剂量不规范使用，易导致动物性食品中GEN残留超标，对人体健康构成危害，对脑神经、听觉以及肾脏的损害严重。现有的检测庆大霉素方法如HPLC或GC-MS用技术其样品前处理复杂、所需仪器昂贵、操作复杂、耗费时间。而使用庆大霉素ELISA试剂盒能够既快速又准确的分析样品中庆大霉素残留。
本试剂盒是应用ELISA技术研发而成的检测产品，操作时间仅需50分钟，试剂盒灵敏度为0.3ppb，线性范围为0.3ppb～24.3ppb。
【实验原理】
本试剂盒采用间接竞争一步法。在酶标板中同时加入标准品（或样本）、酶标二抗及庆大霉素抗体，酶标板上包被的抗原与加入的标准品（或样本）中的抗原竞争结合加入的庆大霉素抗体，同时酶标二抗与庆大霉素抗体结合。用TMB底物显色，样本吸光值与其残留物庆大霉素浓度呈负相关，与校准曲线比较再乘以其对应的稀释倍数，即可得出样本中庆大霉素的含量。
【产品性能指标】
1、检测范围：0.3ppb～24.3ppb
2、灵敏度：0.3ppb
精密度：批内差CV%<10%，批间差CV%<10%
4、交叉反应率
5、 回收率
6、样本最低检测限
 
维士康作为武汉华美生物旗下的食品安全健康专家，专注于食品安全领域相关的试剂、设备和监管溯源平台的研发、生产及销售。目前，公司生产及销售的产品种类众多，配套服务完善，在业内享有较好的声誉和口碑。同时，也为食品安全的监督监管和执法提供了有效的协助和保障。
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viral transcription and RNA synthesis by interacting with the viral polymerase L. Presumably recruits the NP encapsidated genome to cellular membranes at budding sites via direct interaction with NP. Plays critical roles in the final steps of viral release by interacting with host TSG101, a member of the vacuolar protein-sorting pathway and using other cellular host proteins involved in vesicle formation pathway. The budding of the virus progeny occurs after association of protein Z with the viral glycoprotein complex SSP-GP1-GP2 at the cell periphery, step that requires myristoylation of protein Z. Also selectively represses protein production by associating with host eIF4E.

Subunit:
Interacts with protein NP; this interaction probablydirects the encapsidated genome to budding sites. Interacts (via RING domain) with polymerase L; this interactioninhibits viral transcription and replication (By similarity).Interacts with the glycoprotein complex; this interaction plays arole in virion budding. Interacts with host eIF4E; this interactionresults in eIF4E reduced affinity for its substrate, the 5'-m7 Gcap structure. Interacts (via late-budding domain) with hostTSG101; this interaction is essential for budding and release ofviral particles. Interacts with host RPLP0; this interaction mayserve to load ribosome-like particles inside the virion. Interactswith host PML; this interaction induces PML bodies redistributionin the cytoplasm upon viral infection.

LCMV RING finger protein Z,淋巴细胞性脉络丛脑膜炎病毒蛋白抗体Subcellular Location:
Virion. Host cytoplasm, host perinuclearregion. Host cell membrane; Lipid-anchor; Cytoplasmic side.Note=Mainly perinuclear. During budding, associates at the innerside of the plasma membrane of infected cells.

Similarity:
Belongs to the arenaviridae Z protein family.
Contains 1 RING-type zinc finger.

Database links:
UniProtKB/Swiss-Prot: P18541

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.