QuantiBRITE Reagents 荧光分子定量相关试剂 | Catalog Number | 341004 | | Brand | BD Quantibrite™ | | Name | CD8/CD38/CD3 | | Size | 50 tests | | Regulatory Status | RUO (GMP) | | Catalog Number | 341004 |
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| SK1 | FITC | Mouse IgG1, k | |
| HB7 | PE | Mouse IgG1, k | |
| SK7 | PerCP | Mouse IgG1, k | |
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| CD8 recognizes an antigen expressed on the 32-kilodalton (kd) a subunit of a disulfide-linked bimolecular complex. The cytoplasmic domain of the a subunit of the CD8 antigen is associated with the protein tyrosine kinase p56lck. The CD8 molecule interacts with class I major histocompatibility complex (MHC) molecules resulting in increased adhesion between the CD8+ T lymphocytes and the target cells. Binding of the CD8 molecule to class I MHC molecules enhances the activation of resting T lymphocytes.CD38 recognizes an integral membrane glycoprotein, Mr 45 kd, with a protein core of 35 kd.CD3 recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20-30 kd. The antigen recognized by the CD3 antibody is noncovalently associated with either a/b or g/d TCR (70-90 kd).The CD8 antigen is expressed on the human suppressor/cytotoxic T-lymphocyte subset (CD3+CD8+), as well as on a subset of natural killer (NK) lymphocytes.The CD38 antigen is expressed on essentially all pre-B lymphocytes, plasma cells, and thymocytes. It is also present on activated T lymphocytes, NK lymphocytes, myeloblasts, and erythroblasts. The antigen is expressed during the early stages of T- and B-lymphocyte differentiation, is lost during the intermediate stages of maturation, and then reappears during the final stages of maturation.In HIV infection, the coexpression of the CD8 and CD38 antigens, with a concomitant low percentage of CD4+ lymphocytes, is closely associated with disease progression. Beginning at seroconversion, and during the first stages of HIV infection, the number of CD8+ T lymphocytes increases while the number of CD4+ T lymphocytes decreases. When the onset of acquired immune deficiency syndrome (AIDS) occurs, there is a decline in the absolute CD3+, CD4+, and CD8+ lymphocyte levels along with a further decrease in the CD4+ T-lymphocyte percentage. The relative percentage of CD8+ T lymphocytes continues to rise until, in the late stages of AIDS, the majority of the remaining T lymphocytes are CD8+. Elevated levels of CD8+CD38+ T lymphocytes become apparent at the time of seroconversion and continue to rise throughout the course of the disease, indicating persistent stimulation of the immune system by the virus.The CD3 antigen is expressed on 61-85% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and on Purkinje cells in the cerebellum. |
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