Background: Fascin is an ~58 kDa monomeric actin filament bundling protein. It is required to maximally cross-link the actin filaments into straight, compact, and rigid bundles, and contributes to the formation of filopodia that are critical for cell migration. Elevated levels of fascin have been found in metastatic tumors and are correlated with clinically aggressive phenotypes, poor prognosis, and shorter survival.
Immunogen: A peptide from the middle of human Fascin 1
Applications: ELISA, WB, IP, IHC
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:2000 IP: 1 µ for 1~2 mg total cellular proteins IHC: 1:100 dilution
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-fascin antibody recognizes fascin 1, 2 and 3 of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Immunofluorescence:
Detection of fascin by immunofluorescence in 4T1 mouse breast tumor cells with antifascin monoclonal antibody (Cat # 26013).