anti-GRB7 Mouse Monoclonal Antibody
英文名称: anti-GRB7 Mouse Monoclonal Antibody
型号:null    抗体货号: 26133
价格:请致电:010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

GRB7 [26133]
[100 µL]
 

Catalog Number:  26133
Gene Symbol:  GRB7
Description:   anti-GRB7 Mouse Monoclonal Antibody
Background:   Grb7 is an adaptor protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. It plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Grb7 gene is commonly co-amplified (present in excess copies) in breast cancers. Grb7 is over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers. There are clues that it involved in cell migration and metastatic progression of human esophageal carcinomas.
Immunogen:   Purified GRB7 protein, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200
Concentration:    0.5 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes GRB7 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant GRB7 protein. Purified His-tagged GRB7 protein was blotted with anti-GRB7 mouse monoclonal antibody (Cat. #26133).
Immunohistochemistry:  

 

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-GRB7 monoclonal antibody (Cat. #26133). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.