Background
The ZytoFast ® EBV-CISH System is desig-ned for the detection of Epstein-Barr virus (EBV) RNA in paraffin-embedded tissue sections or cell samples.
EBV (a.k.a. human herpesvirus-4, HHV-4) is a member of the gamma-herpesvirus group and one of the most common viru-ses in humans.
Transmission of EBV requires close, intima-te contact with a person excreting the virus in its saliva. EBV has two major target tissues in vivo, B lymphocytes and squa-mous pharyngeal epithelium. Infection of B lymphocytes with EBV results in persistant latent infection, immortalization of the cells, and perpetual proliferation. EBV, the first virus to be identified as an oncovirus, is the etiological agent of infectious mononucleosis and has been implicated in the pathogenesis of an increasing number of human malignancies such as Burkitt’s lymphoma, nasopharyngeal carcinoma, and polyclonal lymphomas in immuno-compromised individuals. CISH-based diagnosis of EBV infection has the advan-tage over other methods in that it permits unequivocal localization of EBV genomes in cells and thereby obviates the risk of false positive results due to laboratory or clinical contamination.

Probe Description
The ZytoFast ® EBV Probe is directed against EBER-1 and EBER-2 RNA sequen-ces that were found to be transcribed in every latently infected cell. Due to the large number (up to 107) of copies per cell, these RNAs are the most abundant transcripts in latently EBV-infected cells. The probe consists of EBV-specific oligonu-cleotides, Biotin or Digoxigenin labeled by using the unique ZytoFast ® High Tag Sys-tem providing improved signal intensity.

Results
A positive reactivity for Epstein-Barr-Virus (EBV) EBER RNA in the target cells is indi-cated by a distinctly stained nucleus. De-pending on the detection chemistry that is used, colored precipitates, which can be clearly distinguished from the background, will be dark violet-blue when using NBT/BCIP as substrate, strong red when using AEC, or dark brown when using DAB.


采购指南

1、ZytoFast EBV-CISH Probes
      Biotin-labeled

T-1014-400 ZytoFast EBV Probe 40 (400μl)

相关产品：
T-1070-40 ZytoFast CISH Implementation Kit AP-NBT/BCIP 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Pepsin Solution, 4 ml; 20x Wash Buffer TBS, 2x 50 ml;
AP-Streptavidin, 4 ml; NBT/BCIP, 4 ml

T-1071-40 ZytoFast CISH Implementation Kit HRP-AEC 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Pepsin Solution, 4 ml; 20x Wash Buffer TBS, 2x 50 ml;
HRP-Streptavidin, 4 ml; AEC Solution, 4 ml


2、ZytoFast ® EBV-CISH Probes
     Digoxigenin-labeled

T-1114-400 ZytoFast EBV Probe 40 (400 μl)

相关产品：
T-1061-40 ZytoFast PLUS CISH Implementation Kit AP-NBT/BCIP 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;20x Wash Buffer TBS, 4x 50 ml; Rabbit-anti-DIG, 4 ml; Anti-Rabbit-AP-Polymer, 4 ml; NBT/BCIP, 4ml; Nuclear Red Solution, 20 ml; Mounting Solution (alcoholic), 4 ml

T-1062-40 ZytoFast PLUS CISH Implementation Kit HRP-AEC 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;20x Wash Buffer TBS, 4x 50 ml; Mouse-anti-DIG, 4 ml; Anti-Mouse-HRP-Polymer, 4 ml; AEC Solution, 4 ml; Nuclear Blue Solution, 20 ml; Mounting Solution (aqueous), 4 ml

T-1063-40 ZytoFast PLUS CISH Implementation Kit HRP-DAB 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;20x Wash Buffer TBS, 4x 50 ml; Mouse-anti-DIG, 4 ml; Anti-Mouse-HRP-Polymer, 4 ml; DAB Solution A, 0.3 ml; DAB Solution B, 10 ml; Nuclear Blue Solution, 20 ml; Mounting Solution (alcoholic), 4 ml

T-1073-40 ZytoFast PLUS CISH Implementation Kit HRP-HRP-Green 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 4x 50 ml; Mouse-anti-DIG, 4 ml; Anti-Mouse-HRP-Polymer, 4 ml; HRP-Green-Solution A, 0.8 ml; HRP-Green-Solution B, 15 ml; Nuclear Red Solution, 20 ml; Mounting Solution (alcoholic), 4 ml

T-1151-40 ZytoFast PLUS CISH Implementation Kit AP-Permanent Red 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 4x 50 ml; Rabbit-anti-DIG, 4 ml; Anti-Rabbit-AP-Polymer, 4 ml; Permanent Red Solution A, 0.2 ml; Permanent Red Solution B, 14 ml; Mayer’s Hematoxylin Solution, 20 ml;
Mounting Solution (alcoholic), 4 ml