产品名称
Anti-Collagen I抗体
描述
兔多克隆抗体to Collagen I
特异性
Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody. Abcam does not test ab34710 with endogenous samples in WB.
经测试应用
适用于: IHC-Fr, Indirect ELISA, WB, IHC-P, ELISA, ICC/IF, IPmore details
种属反应性
与反应: Mouse, Rat, Goat, Horse, Cow, Human, Pig, Common marmoset
免疫原
Full length native protein (purified) corresponding to Human Collagen I aa 1-1464.
Database link: P02452
阳性对照
Wistar Rat hepatic stellate cell lysate [GFP transduced], for Western blotting. IHC-P: Human Placenta Normal.
常规说明
It is often extremely difficult to generate antibodies with specificities to collagens due to the uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes - this may result in diminished reactivity of some antibodies with denatured collagen or formalin-fixed, paraffin embedded tissues. Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis.
形式
Liquid
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液
Preservative: 0.01% Sodium Azide
Constituents: 0.125M Sodium borate, 0.075M Sodium chloride, 0.005M EDTA. pH 8.0
浓度
100 µg 浓度为 1 mg/ml
纯度
Immunogen affinity purified
纯化说明
This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Sterile filtered.