Description

The development of the cerebellum involves a set of coordinated cell movements and two separate proliferation zones: the ventricular zone and the external granule cell layer (EGL), a rhombic-lip-derived progenitor pool [1]. The EGL appears to be segregated during early cerebellum formation and produces only granule cells. Cerebellar granule cells (CGC) are the most abundant neurons of the brain; about 101 billion in man [2]. Their axons run as parallel fibres along the coronal axis, and the one-dimensional spread of excitation that is expected to result from this arrangement is a key assumption in theories of cerebellar function. CGC receive inhibitory synaptic input from Golgi cells, which are mediated by gamma-aminobutyric acid (GABA). During both in vivo and in vitro development, CGC depend on the activity of the NMDA glutamate receptor subtype for survival and full differentiation [3]. Cultured CGC are widely used as a model system for studying neuronal apoptosis.

HCGC from ScienCell Research Laboratories are isolated from human cerebellum. HCGC are cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HCGC are characterized by immunofluorescent method with antibodies to neurafilament, MAP2, and beta-tubulin 3. HCGC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCGC are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Neuronal Medium (NM, Cat. No. 1521) for the culture of HCGC in vitro.

Product Use

HCGC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1] Hatten, M. E. (1999) Central nervous system neuronal migration. Annu. Rev. Neurosci. 22, pp. 511-539.
[2] Andersen, B.B., Korbo, L. and Pakkenberg, B. (1992) A quantitative study of the human cerebellum with unbiased stereological techniques. J. Comp. Neurol., 326:549-560.
[3] Monti, B, Marri, L, Contestabile, A. (2002) NMDA receptor-dependent CREB activation in survival of cerebellar granule cells during in vivo and in vitro development. Eur J Neurosci. 16:1490-8.

    1000 人脑微血管内皮细胞 (HBMEC) ( 5×105 )
    1100 人脑血管平滑肌细胞 (HBVSMC)( 5×105 )
    1200 人脑血管周细胞 (HBVP) ( 5×105 )
    1300 人脉络丝内皮细胞 (HCPEC)( 5×105 )
    1310 人脉络丝表皮细胞 (HCPEpiC)( 5×105 )
    1320 人脉络丝成纤维细胞 (HCPF)( 5×105 )
    1400 人脑膜细胞 (HMC)( 5×105 )
    1520 人神经细胞（HN) (10×106 )
    1530 人小脑颗粒细胞 (HCGC)( 5×105 )
    1540 人海马趾神经细胞(HN-h)(1×106)
    1600 人少突胶质前体细胞（HOPC）（贴壁生长）
    1610 人少突胶质前体细胞（HOPC-os）（悬浮生长）
    1620 人胶质细胞(少突细胞)(HO) (1×106 )
    1700 人雪旺细胞 ( HSC) ( 5×105 )
    1710 人神经系统周边细胞 (HPNC)( 5×105 )
    1800 人星形胶质细胞 (HA) (1×106 )
    1810 人小脑星形胶质细胞( Hac) (1×106 )
    1820 人脊髓星形胶质细胞(HA-sp)(1×106)
    1830 人海马星形胶质细胞(HA-h)(1×106)
    1900 人小神经胶质细胞(HM) ( 5×105 )

Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应

       近几年的新药研发成功率在逐年下降，其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利用这些原代细胞株所甄别和筛选出来的候选药物，其诊治人类疾病的成功几率将大大增加。这样不但大大节省了新药的开发成本，而且将极大地提高人类的健康质量。这些产品从根本上提高了全球生命医学研究、人类重要疾病药物研发、新药研发的成功率。所以上海中乔新舟生物科技有限公司致力于提供优质原代细胞产品和完善的售后服务。

       美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。

        ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的真实性、重复性和连贯性。

Sciencell公司部分原代细胞目录（如需要其他细胞资料请发邮件至 wwwfudan@163.com索取）：

        中乔新舟www.zqxzbio.com专长于为生物医药领域的医疗机构、研究中心、企业、临床医生等提供课题设计、基金联合申请、实验技术服务、论文相关服务、采购外包等整体服务，目前已经成长为国内领先的转化医学外包品牌。
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