上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理: 美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。 ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。确保了实验结果的重复性和连贯性。 请登录Sciencell公司官方网站(www.zqxzbio.com或www.sciencellonline.com)以确保购买正规公司产品。  人主动脉平滑肌细胞说明: 货号:6110 平滑肌细胞是多数重要动脉疾病的靶细胞。血管SMC的生长潜力是原发血管病发中至关重要的异常因素。最新研究表明SMC表达的钙通道,及其表面表达的ICAM-1和VCAM-1,均参与了血管壁的炎症反应,并与血管疾病的进展和稳定有关。人血管平滑肌的体外培养可作为血管研究模型,为血管疾病的治疗和药理学研究提供信息,发挥重要作用。 人主动脉平滑肌细胞(HASMC)提取于人大动脉组织,原代冻存。每管含有细胞数>5×105cells/ml,此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证,经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。 推荐培养基:(SMCM, Cat. No. 1101) | | Description Smooth muscle cell (SMC) is the cellular substate of most significant arterial disease [1]. The increased growth potential of vascular SMC represents one of the crucial anomalies responsible for the development of essential vascular diseases. New studies demonstrate that SMC express calcium channels [2] and the expression of ICAM-1 and VCAM-1 on SMC may contribute to the inflammatory reaction in the vascular wall and may actively be involved in the progression and stability of vascular disease [3]. In vitro culture of human vascular SMC as a model of vascular research played a critical role and continues providing information in the pharmacology and the therapy of vascular diseases. HASMC from ScienCell Research Laboratories are isolated from human aorta. HASMC are cryopreserved at secondary culture after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HASMC are characterized by immunofluorescent method with antibodies to ?-smooth muscle actin and desmin. HASMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HASMC are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. No. 1101) for the culturing of HASMC in vitro. Product Use HASMC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. Shipping Dry ice. Reference [1] Schwartz, S. M., Campbell, G. R., Campbell, J. H. (1986) Replication of smooth muscle cells in vascular disease. Circ. Res. 58:427-444. [2] Fan, Q. I., Vanderpool, K., Marsh, J. D. (2002) A 27 bp cis-acting sequence is essential for L-type calcium channel alpha(1C) subunit expression in vascular smooth muscle cells. Biochim Biophys Acta. 1577:401-11. [3] Braun, M., Pietsch, P., Schror, K., Baumann, G., Felix, S. B. (1999) Cellular adhesion molecules on vascular smooth muscle cells. Cardiovasc. Res. 41:395-401. | 14.心脏细胞系统 6000 人心脏微血管内皮细胞 (HCMEC)( 5×105 ) 6100 人主动脉内皮细胞 (HAEC)( 5×105 ) 6110 人主动脉平滑肌细胞 (HASMC)( 5×105 ) 6200 人心肌细胞 (HCM) (1×106 ) 6210 ***心肌细胞 (HCMa) (1×106 ) 6300 人心脏成纤维细胞( HCF) ( 5×105 ) 6310 ***心室成纤维细胞 (HCFav)( 5×105 ) 6320 ***心房成纤维细胞 (HCFaa)( 5×105 ) |