上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理: 美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。 ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。确保了实验结果的重复性和连贯性。 请登录Sciencell公司官方网站(www.zqxzbio.com或www.sciencellonline.com)以确保购买正规公司产品。 人心肌细胞--***说明: 货号:6210 心肌细胞是机体上最具有活力的细胞。它的收缩是肌原性的,其活动与神经刺激无关。所有的心肌细胞都能自律性的去极化和复极化。心肌细胞占有心脏重量的75%,但仅为心脏细胞数量的1/3。它们是高度特异的具有高氧含量且含有大量的线粒体的细胞。已分化的心肌细胞没有增殖能力,它们在a-***的刺激下通过Ras/MEK途径发生肥大生长。心力衰竭时,心肌细胞的肥大和凋亡表示心肌收缩功能的下降。心肌细胞具有复杂的信号网络,从而调节其心脏节律性的泵出功能中的作用。该信号网络是研究心肌细胞死亡的细胞信号机理的模型。 人心肌细胞(HCM)提取于人心脏组织,提取纯化之后立即冻存。每管含有细胞数>5 x 105 cells/ml,此细胞通过Myosin免疫荧光染色验证,经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。 推荐培养基:(CMM, Cat. No. 6201) | | Description The cardiac myocyte is the most physically energetic cell in the body. Its contraction is myogenic, i.e. it is independent of nervous stimulation. All cardiac myocytes are capable of spontaneous rhythmic depolarization and repolarization of their membrane. Cardiac myocytes occupy as much as 75% of cardiac mass but constitute only about one third of the total cell number in the heart. They are highly specialized high-oxygen-content cells and house a large number of mitochondria [1]. Differentiated cardiac myocytes have little capacity to proliferate and show the hypertrophic growth in response to alpha1-adrenergic stimuli via the Ras/MEK pathway [2]. Cardiac myocyte hypertrophy and apoptosis have been implicated in the loss of contractile function during heart failure. Cardiac myocytes have a complex network of signals that regulates their essential role in the rhythmic pumping of the heart [3]. This network is an appealing model system in which to study the basic principles of cellular signaling mechanisms leading to cardiac myocyte death. HCMa from ScienCell Research Laboratories are isolated from human heart tissue. HCMa are cryopreserved immediately after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCMa are characterized by immunofluorescent method with antibodies to myosin. HCMa are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCMa are guaranteed to further culture at the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Cardiac Myocyte Medium (CMM, Cat. No. 6201) for the culturing of HCMa in vitro. Product Use HCMa are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. Shipping Dry ice. Reference [1] Bodyak, N., Kang, P. M., Hiromura, M., Sulijoadikusumo, I., Horikoshi, N., Khrapko, K. and Usheva, A. (2002) Gene expression profiling of the aging mouse cardiac myocytes. Nucleic Acids Research 30(17):3788-3794. [2] Tamamori-Adachi, M., Ito, H., Nobori, K., Hayashida, K., Kawauchi, J., Adachi, S., Ikeda, M. A. and Kitajima, S. (2002) Expression of cyclin D1 and CDK4 causes hypertrophic growth of cardiomyocytes in culture: a possible implication for cardiac hypertrophy. Biochem Biophys Res Commun 296(2):274-80. [3] Sambrano, G.R., Fraser, I., Han, H., Ni, Y., OConnell, T., Yan, Z. and Stull, J. T. (2002) Navigating the signaling network in mouse cardiac myocytes. Nature 420(6916):712-4. | 14.心脏细胞系统 6000 人心脏微血管内皮细胞 (HCMEC)( 5×105 ) 6100 人主动脉内皮细胞 (HAEC)( 5×105 ) 6110 人主动脉平滑肌细胞 (HASMC)( 5×105 ) 6200 人心肌细胞 (HCM) (1×106 ) 6210 ***心肌细胞 (HCMa) (1×106 ) 6300 人心脏成纤维细胞( HCF) ( 5×105 ) 6310 ***心室成纤维细胞 (HCFav)( 5×105 ) 6320 ***心房成纤维细胞 (HCFaa)( 5×105 ) |