兔抗单核细胞抗体(AMA)ELISA试剂盒
英文名称: Rabbit anti-Monocyte antibody,AMA ELISA
型号:null
价格:请致电:010-57128832,18610462672
品牌: 中国
试剂级别: 生化级

样本处理及要求:1. 血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。2. 血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。3. 尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。4. 细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。5. 组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。6. 标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.7. 不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。本产品仅供科研使用欢迎来电咨询详细产品说明书。 

Size/Quantity 10 mg/ml; 5 x 1 ml Product Use Recommended concentration range: 5-100 ug/ml. The final working concentration, however, should be optimized for individual cell type and culture conditions. Note: Color may vary between lots and lipoprotein precipitates may be present. This does not affect the biological activity. BHE is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage Store BHE between -20°C and -80°C until ready for use. Aliquot to avoid repeated freeze-thaw cycles Shipping Info Dry ice. References [1] Maciag T, Cerundolo J, Ilsley S, Kelley PR, Forand R. (1979) "An endothelial cell growth factor from bovine hypothalamus: Identification and partial characterization". Proc. Natl. Acad. Sci. USA 76: 5674-5678. [2] Gilchrest BA, Calhoun JK, Maciag T. (1982) "Attachment and growth of human keratinovytes in a serum-free environment". Journal of Cellular Physiology 112: 197-206. [3] Maciag T, Nemore RE, Weinstein R, Gilchrest BA. (1981) "An endocrine approach to the control of epidermal growth: serum-free cultivation of human keratinocytes". Science 211: 1452-1454. [4] Pintus C, Ransom JH, Evans CH. (1983) "Endothelial cell growth supplement: a cell cloning factor that promotes the growth of monoclonal antibody producing hybridoma cells". J Immunol Methods 61: 195-200. 

Caution: If handled improperly, some components of this product may present a health hazard. Take appropriate precautions when handling this product, including the wearing of protective clothing and eyewear. Dispose of properly