Murine & Rat Growth Factors & Cytokines
Recombinant Rat IFN-gamma


 
 
Synonyms :
Immune Interferon, type II interferon, T cell interferon, MAF
Description :
IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma is species-specific and is biologically active only in human and primate cells. Recombinant rat IFN-gamma is a 15.6 kDa protein containing 135 amino acid residues.
Catalog #:
400-20
Source :
E.coli
Purity :
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin Level :
Endotoxin level is less than 0.1 ng per μg (IEU/μg).
Biological Activity :
The ED50 as determined by a cytopathic affect inhibition assay with murine L929 cells challenged with EMC virus was < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
Selected References :
Selected List of References Citing This PeproTech Product
AA Sequence :
MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC 
PubMed Link :
IFN-gamma+AND+rat
Country of Origin :
USA
 
 
Size A
20μg
$80 
 
 
Size B
100μg
$195 
 
 
Size C
1mg
$1100 
 
 

六、常见产品问题汇总
1.  Q：哪些细胞系适合用yocon无血清细胞冻存液冻存? 
    A：理论讲所有细胞冻存都适用 ，但细胞系种类繁多，无法逐一验证，需要客户冻存前进行预实验。
2.  Q：我用我的冻存程序习惯了，可以还继续用程序降温的程序吗？ 
    A：虽然我们产品可以无需程序降温冻存细胞，但如果用传统方法冻存的话也没有问题。对比复苏率一致，无明显差别。
3.  Q：冻存的细胞复苏后如不离心，会有什么后果 ？ 
    A：某些细胞对冻存液中的保护剂较敏感，如复苏时不离心，需传1-2代才可恢复。
4.  Q：无血清冻存液与传统冻存液的冻存原则一样吗？ 
    A：有些区别，主要在冻存步骤上，传统冻存液需要程序降温，4℃ ，-20℃ 、-80℃、液氮；而无血清冻存液无需程序降温，-80℃12h后直接投入液氮。两者复苏程序一致，要保证快复苏的原则。

七、产品质控指标
PH值：7.0-8.5
渗透压：1600-2000mOsm/Kg 
无菌：霉菌、细菌不得检出
装量：100ml/瓶
外观:橘红色澄清液体
保存温度：2-8度，避光保存

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