Recombinant Human IFN-γ
Catalog Number:
AF-300-02
Synonyms:
Immune Interferon, type II interferon, T cell interferon, MAF
Description:
IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-γ species-specific and is biologically active only in human and primate cells. Recombinant human IFN-γ is a 16.7 kDa protein containing 143 amino acid residues. Manufactured using all non-animal reagents.
AA Sequence:
MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ
Source:
E.coli
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml.
Assay #2: The ED50 was determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
Assay #2: The ED50 was determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
Cross Reactivity:
Monkey
Research Interests:
 | AIDS / HIV |  | Angiogenesis/ Cardiovascular |
 | Cancer |  | Diabetes / Weight Regulation |
 | Immune System |  | Neurobiology |
 | Stem Cells & Differentiation |  | Allergy |
 | Transplantation | ||
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.