Monoclonal Antibody against BRST-1
英文名称: Monoclonal Antibody against BRST-1
型号:null    产品货号: SIG-3610
价格:请致电:010-57128832,18610462672
品牌: usa

DescriptionBRST-1 (CU-18) Monoclonal Antibody (SIGNET) - SIGNET ANTIBODIESMonoclonal Antibody against BRST-1 (CU-18)
(Formerly Signet Catalog Nos: 610-01, 610-16 L1 Predilute, 610-26 L2 Predilute, 610-36 LV Predilute)
Intended Use** In Vitro Diagnostic (IVD) **
CloneCU-18
FormTissue Culture Supernatant (in 0.01M PBS + 0.1% NaN3 + 1% BSA)
HostMouse
Species ReactivityHuman
IsoTypeIgG1
SpecificityBRST-1 (CU-18) Monoclonal Antibody (SIGNET) - SIGNET ANTIBODIESThe BRST-1, clone CU-18, monoclonal antibody detects BCA-225, a 225kDa glycoprotein secreted by the T47D breast carcinoma cell line. Strong intracytoplasminc staining is seen in primary and metastatic breast carcinoma cell lines, primary and metastatic breast carcinoma tissue and cervical carcinomas.

This antibody was generated against a protein secreted by the T47D (Clone 11) human breast carcinoma cell line.

Concentrated Format:
SIG-3610-1000 [1 mL]

Prediluted Formats:
SIG-3610-16 [6 mL] is ready to use with Peroxidase Anti- Peroxidase detection systems.

SIG-3610-26 [6 mL] is ready-to-use with Biotin-based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).

SIG-3610-36 [6 mL] is ready-to-use with Biotin based detection systems on Ventana ES and Ventana NexES automated immunostainers.
UsesBRST-1 (CU-18) Monoclonal Antibody (SIGNET) - SIGNET ANTIBODIESThis antibody is effective in immunohistochemistry (IHC).
Suggested Working DilutionBRST-1 (CU-18) Monoclonal Antibody (SIGNET) - SIGNET ANTIBODIESThe optimal working dilution should be determined for each specific assay condition.
  • IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
    Tissue Sections: Formalin-fixed, paraffin-embedded tissues
    Pretreatment: Not required
    Incubation: 20 minutes at room temperature
NotesBRST-1 (CU-18) Monoclonal Antibody (SIGNET) - SIGNET ANTIBODIESPositive tissue (human): Breast carcinoma
StorageBRST-1 (CU-18) Monoclonal Antibody (SIGNET) - SIGNET ANTIBODIESStore between 2-8°C.
ReferencesImam SA, et al. Comparison of two microwave based antigen retrieval solutions in unmasking epitopes in formalin-fixed tissue for immunostaining. Anticancer Res 15:1153-8, 1995.

Ansai S, et al. An immunohistochemical study of BCA-225 in various skin cancers. J Dermatol 21(1): 20-4, 1994.

Ma CK, et al. Comparative immunohistochemical study of primary and metastatic carcinomas of the liver. Am J Clin Pathol 99(5):551-7, 1993.
Warranty/ConditionsBRST-1 (CU-18) Monoclonal Antibody (SIGNET) - SIGNET ANTIBODIESCovance products may not be resold or modified for resale without prior written approval.
Rev. DateBRST-1 (CU-18) Monoclonal Antibody (SIGNET) - SIGNET ANTIBODIES3/17/2009