Affinity Matrix: Monoclonal Mouse Antibody, AU1
英文名称: Affinity Matrix: Monoclonal Mouse Antibody, AU1
型号:null    产品货号: AFC-130P
价格:请致电:010-57128832,18610462672
品牌: usa

DescriptionAU1 Monoclonal Antibody, Affinity Matrix - EPITOPE TAGSAffinity Matrix: Monoclonal Mouse Antibody, AU1
Intended Use**Research Use Only (RUO)**

This product is sold for laboratory research use only, not for human or in-vivo use.
CloneAU1
FormPurified IgG immobilized on Sepharose™ Fast Flow beads (in PBS + 0.03% Thimerosal)
[Ab]AU1 Monoclonal Antibody, Affinity Matrix - EPITOPE TAGS2 mg/mL
SpecificityAU1 Monoclonal Antibody, Affinity Matrix - EPITOPE TAGSMonoclonal mouse antibody AU1 recognizes the peptide epitope, DTYRYI. This antibody was purified using protein-A chromatography, and was subsequently immobilized onto a Sepharose™ Fast Flow matrix.
UsesAU1 Monoclonal Antibody, Affinity Matrix - EPITOPE TAGSThis affinity matrix can be used for immunopurification of AU1 tagged fusion proteins from crude starting material. On an analytical scale, it can also be used for immunoprecipitating AU1 tagged proteins.
Suggested Working DilutionAU1 Monoclonal Antibody, Affinity Matrix - EPITOPE TAGSThe optimal buffers and matrix concentration should be determined for each specific assay condition.

Binding: Tagged protein will bind to matrix in common physiologic buffers with pH in the range of 6.0-7.5, salt from 50-500 mM and in the presence of reasonable levels of detergent. Excess reducing agent should be avoided as the disulfide bridges holding antibody heavy and light chains may be compromised. Covance tests Affinity Matrix using an equilibration/binding buffer containing:
  • 100 mM Tris-HCl (pH 7.5)
  • 150 mM NaCl
  • 0.1% Tween 20
  • 0.5% BSA
  • 1 mM beta-mercaptoethanol


Washing: After binding, washes with several bead volumes of buffer are recommended. Such buffer may contain increased salt, altered pH, etc. as determined empirically to remove un-tagged proteins.

Elution: Several options are available for elution.
1. SDS gel loading buffer may be applied directly to the beads in order to display all bound protein on a polyacrylamide gel/western. Note that gel loading buffer containing reducing agent will also release some antibody heavy and light chains (approx 25 and 50kD, respectively).
2. Competitive elution with epitope peptide. For epitope tag affinity matrices, prepare an elution buffer with epitope tag peptide at 400 ug/mL in 50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM EDTA (pH 8.0).
3. Chemical Elution. Elution by pH or chaotropic salts is also possible. For elution by pH, either 0.1 M glycine pH 2.8 or 40 mM diethyl-amine pH 11.0 may be used.
NotesAU1 Monoclonal Antibody, Affinity Matrix - EPITOPE TAGSSepharose is a trademark of Amersham Biosciences Limited
StorageAU1 Monoclonal Antibody, Affinity Matrix - EPITOPE TAGSThe matrix may be re-used several times. To strip column after use, wash with several bead volumes 0.1 M glycine pH 2.8 followed immediately by PBS cotaining 0.3% thimerosol or 1mM azide as preservative. Store at 4°C. This shelf-life of this product is approximately 1 year.
ReferencesField J, Nikawa J-I, Broek D, MacDonald D, Rodgers L, Wilson I, Learner R, Wigler M. Purification of RAS responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell Biol 8:2159-2165, 1988.
Warranty/ConditionsAU1 Monoclonal Antibody, Affinity Matrix - EPITOPE TAGSCovance products may not be resold or modified for resale without prior written approval.

188.00

产品描述

制备聚丙烯酰胺凝胶时,常使用Bind-Silane(亲和硅烷)处理长玻璃板,使凝胶与之粘贴。用Repel-Silane(剥离硅烷)处理短玻璃板,使凝胶易于分离。

本品为亲和硅烷。

使用方法

制备聚丙烯酰胺凝胶时,请先将长、短玻璃板用1MNaOH浸泡一段时间,并用洗涤剂清洗一遍,之后用水洗掉洗涤剂,再用去离子水冲洗干净。最后用乙醇擦拭玻璃板。

1 长玻璃板的处理

每次铺胶前均需用亲和硅烷对长玻璃板进行处理。

1)用镜头纸蘸取少许亲和硅烷溶液(1ml左右,视玻璃板大小而定)涂在长玻璃板上,自上而下涂一次,从左至右涂一次,将整块玻璃板涂满,涂匀。

25-10分钟后,用干净的镜头纸轻轻擦拭玻璃板以除去多余的亲和硅烷。

【注】:或用95%的乙醇清洗长玻璃板3次,以去除多余的亲和硅烷。

2短玻璃板的处理

每次铺胶前均需用剥离硅烷对短玻璃板进行硅化处理。

1)处理短玻璃板前必需更换手套,以防手套上的亲和硅烷污染短玻璃板,使短玻璃板也粘胶。

2)用镜头纸蘸取适量剥离硅烷溶液(1.5ml左右,视玻璃板大小而定)涂在短玻璃板上,自上而下涂一次,从左至右涂一次,将整块玻璃板涂满,涂匀。

35-10分钟后,用干净的镜头纸轻轻擦拭玻璃板以除去多余的剥离硅烷。

运输与储存

室温运输和保存即可。有效期1年。

注意事项

1)若需重复使用长玻璃板,可用1MNaOH浸泡过夜,然后用大量清水冲洗。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

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