Description		Monoclonal Antibody against neuron-specifc enolase, clone NSE-P2
Clone		NSE-P2
Form		Purified Antibody
Host		Mouse
IsoType		IgG1
[Ab]		1 mg/mL
Specificity		Neuron specific enolase is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. This antibody was raised against a synthetic peptide corresponding to amino acids 271-285 It recognizes the sequence TGDQLGALYQDFVRD.
Uses		This antibody is effective in ELISA, immunoblotting and immunohistochemistry.
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. Western blot: 1:2,000* ELISA: 1:500
Notes		* Predicted MW = 47 kD
Storage		Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
References		Murray GI, Duncan ME, Melvin WT, Fothergill JE. Immunohistochemistry of neurone specific enolase with gamma subunit specific anti-peptide monoclonal antibodies. J. Clin. Pathol. 46:993-996, 1993. Duncan ME, McAleese SM, Booth NA, Melvin WT, Fothergill JE. A simple enzyme-linked immunosorbent assay (ELISA) for the neuron-specific gamma isozyme of human enolase (NSE) using monoclonal antibodies raised against synthetic peptides corresponding to isozyme sequence differences. J. Immuno. Methods 151:227-236, 1992.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.
Rev. Date		11/7/2006