英文名称： Monoclonal Antibody against Green Fluorescent Protein (GFP)
| Description |  | Monoclonal Antibody against Green Fluorescent Protein (GFP) |
| Clone | | B34 |
| Form | | Purified Antibody (Stored in PBS + 0.03% Thimerosal) |
| Host | | Mouse |
| IsoType | | IgG1 |
| [Ab] | | 1 mg/mL |
| Specificity | | Green Fluorescent Protein (GFP) is a single chain 238 amino acid protein derived from the jellyfish Aequoria victoria. GFP emits a bright green fluorescence when illuminated with UV or blue light and is a useful tool for cellular and developmental biologists. Unlike other bioluminescent molecules, GFP does not require additional proteins, substrates or co-factors for its activity. Antibody B34, generated against purified wild type GFP, offers an alternitive for detecting GFP and GFP variants including enhanced/mutant GFP. |
| Uses | | This antibody is effective in immunoblotting of GFP labeled proteins. |
| Suggested Working Dilution | | The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval.
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| Notes | | *Predicted MW = 27 kD. |
| Storage | | Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
| References | | Pecot MY, Malhotra V. Golgi membranes remain segregated from the endoplasmic reticulum during mitosis in mammalian cells. Cell 116:99–107, 2004. Kessels MM, Engqvist-Goldstein AE, Drubin DG, Qualmann B. Mammalian Abp1, a signal-responsive F-actin-binding protein, links the actin cytoskeleton to endocytosis via the GTPase dynamin. J. Cell. Biol. 153(2):351-366, 2001. Gerdes HH, Kaether C. Green fluorescent protein: applications in cell biology. FEBS Lett. 389:44-47, 1996. Davis I, Girdham CH, O'Farrell PH. A nuclear GFP that marks nuclei in living Drosophila embryos; maternal supply overcomes a delay in the appearance of zygotic fluorescence. Dev. Biol. 170:726-729, 1995. Chalfie M. Green fluorescent protein. Photochem. Photobio. 62:651-656, 1995. Stearns T. Green fluorscent protein. The green revolution. Curr. Biol. 5:262-264, 1995. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. Green fluorescent protein as a marker for gene expression. Science 263:802-805, 1994. |
| Warranty/Conditions | | Covance products may not be resold or modified for resale without prior written approval. |
| Rev. Date | | 2/18/2007 |