Description		Polyclonal Antibody against Glycine Receptor, lyophilized
Form		Lyophilized Antibody (lyophilized from ammonium bicarbonate (5 mM) so some residual salt may be present)
Host		Rabbit
Species Reactivity		Rat
Specificity		Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission. The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin. Gene targeting in mice showed that gephyrin is required for synaptic clustering of glycine receptors in spinal cord. The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins. These receptors have also been extremely useful in studies of synaptic clustering of receptors. During postnatal motoneuron development, the glycine receptor alpha subunit changes from alpha2 (fetal) to alpha1 (adult). This antibody was developed against a peptide derived from the amino terminus of the α1 subunit of Glycine Receptor. It was subsequently purified on an immunogen affinity column.
Uses		This antibody is effective in immunoblotting; specific immunolabeling of the ~48k α1 and α2 subunits of the receptor in rat spinal cord and in cell extracts containing the recombinant human glycine receptors. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen derived from the amino terminus of the α1 subunit of the receptor.
Suggested Working Dilution		The antibody should be reconstituted in 100 µL phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. Western blot:   1:1,000* Dot blot:   1:1,000
Notes		*Predicted MW = 48kD
Storage		The lyophilized product may be stored at -20°C. After reconstitution in PBS, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
References		Schofield CM, Jenkins A, Harrison NL, A highly conserved aspartic acid residue in the signature disulfide loop of the a1 subunit is a determinant of gating in the glycine receptor. J. Biol. Chem. 278:34,079–34,083, 2003. Meier J, Vannier C, Serge A, Triller A, Choquet D. Fast and reversible trapping of surface glycine receptors by gephyrin. Nat. Neurosci. 4:253-260, 2001. Beckstead MJ, Weiner JL, Eger EI, Gong DH, Mihic SJ. Glycine and gammaaminobutyric acid(A) receptor function is enhanced by inhaled drugs of abuse. Mol. Pharmacol. 57:1199-1205, 2000. Eggers ED, O'Brien JA, Berger AJ. Developmental changes in the modulation of synaptic glycine receptors by ethanol. J. Neurophysiol. 84:2409-2416, 2000. Feng GP, Tintrup H, Kirsch J, Nichol MC, Kuhse J, Betz H, Sanes JR. Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity. Science 282:1321-1324, 1998. Froehner SC. Neuroscience - Gathering glycine receptors at synapses. Science 282:1277-1279, 1998. Mihic SJ, Ye Q, Wick MJ, Koltchine VV, Krasowski MD, Finn SA, Mascia MP, Valenzuela CF, Hanson KK, Greenblatt EP, Harris RA, Harrison NL. Sites of alcohol and volatile anaesthetic action on GABA(A) and glycine receptors. Nature 389:385-389, 1997.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.
Rev. Date		11/8/2006