NUTRIENT BROTH

Code: CM0001

A general purpose fluid medium for the cultivation of micro-organisms not exacting in their nutritional requirements. Blood, serum, sugars, etc., may be added as required for special purposes.

Typical Formula*	gm/litre
`Lab-Lemco’ powder	1.0
Yeast extract	2.0
Peptone	5.0
Sodium chloride	5.0
pH 7.4 ± 0.2 @ 25°C

 * Adjusted as required to meet performance standards

Directions
Add 13g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Description
Lab-Lemco beef extract is combined with peptone and sodium chloride to form the basic bouillon described by Loeffler and other early bacteriologists. Yeast extract is added to provide vitamins and minerals to help speed the growth of most organisms.

Nutrient Broth can be enriched with other ingredients such as carbohydrates, blood etc., for special purposes. See also Nutrient Broth No.2 CM0067.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium below 25°C.

Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured solution

Quality control

Positive controls:	Expected results
Staphylococcus aureus ATCC® 25923*	Turbid growth
Escherichia coli ATCC® 25922 *	Turbid growth
Negative control:
Uninoculated medium	No change